Journal ArticleDOI
Detection of Salmonella enterica serovar Typhi (S. Typhi) by selective amplification of invA, viaB, fliC-d and prt genes by polymerase chain reaction in mutiplex format
TLDR
Development of a PCR assay that can target multiple genes for rapid detection of Salmonella enterica serovar Typhi (S. Typhi) from water and food samples is developed.Abstract:
Aims: Development of a PCR assay that can target multiple genes for rapid detection of Salmonella enterica serovar Typhi (S. Typhi) from water and food samples.
Methods and Results: PCR primers for invasion, O, H and Vi antigen genes, invA, prt, fliC-d and viaB were designed and used for the rapid detection of S. Typhi by multiplex PCR. Internal amplification control, which coamplified with prt primers, was also included in the assay. The results showed that all cultures of Salmonella were accurately identified by the assay with no nonspecific amplification in other cultures. The assay had 100% detection probability when a cell suspension of 104 CFU ml−1 (500 CFU per reaction) was used. Salmonella Typhi bacteria were artificially inoculated in the water and food (milk and meat rinse) samples and detected by mPCR after overnight pre-enrichment in buffered peptone water. No Salmonella bacteria could be detected from water samples collected from the field by mPCR or standard culture method.
Conclusions: The developed mPCR assay provides specific detection of S. Typhi.
Significance and Impact of the Study: Rapid methods for detection of S. Typhi from complex environmental matrices are almost nonexistent. The mPCR assay reported in this study can be useful to identify S. Typhi bacteria in field environmental samples.read more
Citations
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Journal ArticleDOI
The Salmonella In Silico Typing Resource (SISTR): An Open Web-Accessible Tool for Rapidly Typing and Subtyping Draft Salmonella Genome Assemblies.
Catherine Yoshida,Peter Kruczkiewicz,Chad R. Laing,Erika J. Lingohr,Victor P. J. Gannon,John H. E. Nash,Eduardo N. Taboada +6 more
TL;DR: It is shown how phylogenetic context from cgMLST analysis can supplement the genoserotyping analysis and increase the accuracy of in silico serovar prediction to over 94.6% on a dataset comprised of 4,188 finished genomes and WGS draft assemblies.
Journal ArticleDOI
PCR multiplex for detection of Salmonella Enteritidis, Typhi and Typhimurium and occurrence in poultry meat
Camila Guimarães de Freitas,Angela Patrícia Santana,Patrícia Helena Caldeira da Silva,Vitor Salvador Picão Gonçalves,Márcia de Aguiar Ferreira Barros,Fernando Araripe Gonçalves Torres,Luci Sayori Murata,Simone Perecmanis +7 more
TL;DR: The conclusion was that the mPCR was able to detect the presence of these bacteria in a short period of time and enabled the identification of serotype Enteritidis in one of the samples found positive for Salmonella sp.
Journal ArticleDOI
Rapid method for detection of Salmonella in milk by surface plasmon resonance (SPR)
TL;DR: The Plasmonic surface plasmon resonance (SPR) device was used to develop a rapid, simple and specific immunoassay for detection of Salmonella in milk, which detected S. typhimurium within 1h, whereas the cultural techniques require 3-4 days for presumptive positive isolates and further time for confirmation.
Journal ArticleDOI
Serotyping, PCR, phage-typing and antibiotic sensitivity testing of Salmonella serovars isolated from urban drinking water supply systems of Nepal
Dwij Raj Bhatta,Dwij Raj Bhatta,Aroon Bangtrakulnonth,P. Tishyadhigama,S.D. Saroj,Jayant R. Bandekar,Rene S. Hendriksen,B.P. Kapadnis +7 more
TL;DR: Aims: To study the occurrence and diversity of Salmonella serovars in urban water supply systems of Nepal.
Journal ArticleDOI
Rapid Genoserotyping Tool for Classification of Salmonella Serovars
Kristyn Franklin,Erika J. Lingohr,Catherine Yoshida,Muna F. Anjum,Levente Bodrossy,Clifford G. Clark,Andrew M. Kropinski,Mohamed A. Karmali +7 more
TL;DR: A Salmonella genoserotyping array which rapidly generates an antigenic formula consistent with the White-Kauffmann-Le Minor scheme, currently the gold standard for Salmoneella serotyping is developed.
References
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Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC, by an enrichment broth culture-multiplex PCR combination assay.
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TL;DR: The scientific journals must provide the source of new PCRbased methods suitable for standardization and henceforward the editorial boards of applied microbiology journals require inclusion of an IAC in diagnostic PCR reported in submitted manuscripts.
Journal ArticleDOI
A Massive Epidemic of Multidrug-Resistant Typhoid Fever in Tajikistan Associated with Consumption of Municipal Water
Jonathan Mermin,Rodrigo G. Villar,Joe Carpenter,Les Roberts,Aliev Samaridden,Larissa Gasanova,Svetlana Lomakina,Cheryl A. Bopp,Lori Hutwagner,Paul S. Mead,Bruce C. Ross,Eric D. Mintz +11 more
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Journal ArticleDOI
Selective Amplification of tyv (rfbE), prt (rfbS), viaB, and fliC Genes by Multiplex PCR for Identification of Salmonella enterica Serovars Typhi and Paratyphi A
Kenji Hirose,Kenichiro Itoh,Hiroshi Nakajima,Takayuki Kurazono,Masanori Yamaguchi,Kazuo Moriya,Takayuki Ezaki,Yoshiaki Kawamura,Kazumichi Tamura,Haruo Watanabe +9 more
TL;DR: The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.
Journal ArticleDOI
Detection of Salmonella typhi by polymerase chain reaction
TL;DR: A pair of PCR primers specific for Salm.