Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method.
Roger A. Warnke,Ronald Levy +1 more
TLDR
In this paper, the binding of the hybridoma monoclonal antibodies to these antigens took advantage of the biotin-avidin interaction, and the detection method employed a biotin conjugate of goat anti-mouse antibody and an avidin conjugates of horseradish peroxidase.Abstract:
Acetone-fixed frozen sections of reactive lymph node, spleen, three B cell lymphomas, and three T cell lymphomas were studied for the presence of Ia-like antigens and two T cell antigens. Detection of the binding of the hybridoma monoclonal antibodies to these antigens took advantage of the biotin-avidin interaction. The detection method employed a biotin conjugate of goat anti-mouse antibody and an avidin conjugate of horseradish peroxidase. B cell lymphoma cells stained for Ia. The cell lymphomas were shown to be heterogeneous for the expression of the two T cell antigens. these three antigens were generally not detectable after 10% formalin fixation of B-5 fixation. Detection of these antigens and the method employed should prove useful in the immunologic categorization of human lymphomas.read more
Citations
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The avidin-biotin complex in bioanalytical applications
Meir Wilchek,Edward A. Bayer +1 more
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Expression of immune system-associated antigens by cells of the human central nervous system: relationship to the pathology of Alzheimer's disease.
TL;DR: The presence of additional immune-related antigens in AD, and distributional differences from normal elderly controls, suggest that the glial proliferation and scavenger activity characteristic of Alzheimer's disease may occur in an immune context and may play an important role in the pathogenesis of the disorder.
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Drug-resistance in multiple myeloma and non-Hodgkin's lymphoma: detection of P-glycoprotein and potential circumvention by addition of verapamil to chemotherapy.
William S. Dalton,Thomas M. Grogan,P.S. Meltzer,Rik J. Scheper,Brian G.M. Durie,Charles W. Taylor,Thomas P. Miller,Sydney E. Salmon +7 more
TL;DR: P-gly expression occurs in drug-refractory B-cell neoplasms and may contribute to the development of clinical drug resistance, however, other factors, such as the proliferative activity of the tumor, may also play a role in determining response to chemotherapy.
Journal ArticleDOI
Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.
Gary S. Wood,Roger A. Warnke +1 more
TL;DR: Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.
Journal ArticleDOI
Infrequent normal B lymphocytes express features of B-chronic lymphocytic leukemia.
TL;DR: Preliminary evidence suggests that at least a subpopulation of these cells is found, in small proportions, within the germinal centers of the normal human tonsil and lymph nodes.
References
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The Use of Avidin-Biotin Interaction in Immunoenzymatic Techniques
TL;DR: Biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested.
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Malignant lymphoma of peripheral T‐lymphocyte origin. Immunologic, pathologic, and clinical features in six patients
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TL;DR: In a continuing study of patients with lymphoproliferative diseases, six adult patients were encountered with a distinctive malignant lymphoma of peripheral T‐lymphocyte origin, with certain characteristic features of the organization of the infiltrate, the morphology of the lymphoid cells, and the nature of non-lymphoid cellular elements.
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A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies
TL;DR: The thymus-leukemia antigen has been isolated from the membranes of leukemia cells by detergent solubilization and subsequent immunoprecipitation with the monoclonal antibody and preliminary biochemical characterization shows the antigen to be associated with a polypeptide of Mr approximately 28,000.
Journal Article
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R. J. Lukes,Clive R. Taylor,J. V. Parker,Thomas L. Lincoln,Paul K. Pattengale,Barbara H. Tindle +5 more
TL;DR: The morphologic features of the cytologic types of the classification of malignant lymphoma of Lukes and Collins are predictive of the subtypes of lymphoma and considerably more effective than the immunologic surface marker techniques in identifying homogeneous groups.
Journal ArticleDOI
Immunologic aspects and pathology of the malignant lymphomas
TL;DR: With new immunologic and cytochemical techniques, it has been possible to characterize normal cells of the T‐lymphocytic, B‐ly mphicetic, and monocyte‐macrophage systems and establish their nature as neoplasms of the immune system.