Development and validation of stability-indicating HPLC method for the simultaneous determination of paracetamol, tizanidine, and diclofenac in pharmaceuticals and human serum.
TLDR
The developed method, after being validated in terms of precision, robustness, recovery, LOD, and LOQ, was successively applied to the analysis of pharmaceutical formulations and human serum.Abstract:
A method is described for the simultaneous determination of paracetamol, tizanidine, and diclofenac in mixtures. The method was based on HPLC separation of the three drugs followed by UV detection at 254 nm. The separation was carried out on a Hypersil ODS, C18 (250 x 4.6 mm id, 10 microm particle size) column using the mobile phase aqueous 0.2% ammonium carbonate-methanol (60 + 40, v/v) at a flow rate of 1 mL/min. The linear regression analysis data were used for the regression curve in the range of 170-10 000 ng/mL for paracetamol, 120-10 000 ng/mL for tizanidine, and 20-10 000 ng/mL for diclofenac. No chromatographic interference from tablet excipients was found. In order to check the selectivity of the proposed method, degradation studies were carried out using hydrolysis (acid, basic, and neutral), thermolysis, and oxidation. The developed method, after being validated in terms of precision, robustness, recovery, LOD, and LOQ, was successively applied to the analysis of pharmaceutical formulations and human serum.read more
Citations
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Analysis of metformin, glimepiride and pioglitazone in human serum and its application to pharmacokinetics
TL;DR: In this article, a robust method for the chromatographic separation of three antidiabetic drugs viz metformin, pioglitazone and glimepiride using an isocratic reversed phase HPLC system having ultraviolet detection at 254 nm is presented.
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Selective Determination of Diclofenac and Clomiphene with a Single Planar Solid-State Potentiometric Ion Selective Electrode
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Simultaneous determination of piroxicam and paracetamol in pharmaceutical formulations using stability indicating hplc method
TL;DR: In this article, a fast, simple, specific, and accurate stability indicating liquid chromatographic method is described for simultaneous determination of piroxicam and paracetamol in bulk drugs and pharmaceutical formulations.
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Simultaneous determination of acetaminophen and dihydrocodeine in human plasma by UPLC-MS/MS: Its pharmacokinetic application.
TL;DR: An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine acetaminophen and dihydrocodeine in human plasma simultaneously and was successfully applied to a pharmacokinetic study of AAP with DHC in Chinese healthy volunteers.
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Development and Validation of HPLC Method for Simultaneous Estimation of Famotidine, Paracetamol and Diclofenac in their Raw Materials and Pharmaceutical Formulation
TL;DR: A simple, sensitive and rapid HPLC method has been developed for simultaneous estimation of a ternary mixture of famotidine, paracetamol and diclofenac in their raw materials and pharmaceutical for.
References
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Book ChapterDOI
Stability Testing of New Drug Substances and Products
TL;DR: In this paper, the authors proposed a method to solve the problem of spamming: http://www.webpages.com.augmentedrome.com/webpages/
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Martindale : the extra pharmacopoeia
TL;DR: Part 1 Monographs on drugs and ancillary substances, Supplementary drugs and other substances, and Indexes - directory of manufacturers.