Journal ArticleDOI
Different nitrogen sources modulate activity but not expression of glutamine synthetase in arbuscular mycorrhizal fungi.
Reads0
Chats0
TLDR
Re-feeding with ammonium produced a general increase in GS activity when compared with hyphae grown in nitrate as a sole N source, and in a N re-supplementation time-course experiment, GS activity responded quickly to addition of nitrate, ammonium or glutamine.About:
This article is published in Fungal Genetics and Biology.The article was published on 2004-05-01. It has received 72 citations till now. The article focuses on the topics: Glutamine synthetase & Glomus.read more
Citations
More filters
Journal ArticleDOI
Nitrogen transfer in the arbuscular mycorrhizal symbiosis
Manjula Govindarajulu,Philip E. Pfeffer,Hairu Jin,Jehad Abubaker,David D. Douds,James Allen,Heike Bücking,Peter J. Lammers,Yair Shachar-Hill +8 more
TL;DR: Results of stable isotope labelling experiments are reported showing that inorganic nitrogen taken up by the fungus outside the roots is incorporated into amino acids, translocated from the extraradical to the intraradical mycelium as arginine, but transferred to the plant without carbon.
Journal ArticleDOI
Carbon availability triggers fungal nitrogen uptake and transport in arbuscular mycorrhizal symbiosis
Carl R. Fellbaum,Emma W. Gachomo,Yugandhar Beesetty,Sulbha Choudhari,Gary D. Strahan,Philip E. Pfeffer,E. Toby Kiers,Heike Bücking +7 more
TL;DR: It is found that the C supply of the host plant triggers the uptake and transport of N in the symbiosis, and that the increase in N transport is orchestrated by changes in fungal gene expression.
Journal ArticleDOI
The uptake, metabolism, transport and transfer of nitrogen in an arbuscular mycorrhizal symbiosis
Hairu Jin,Hairu Jin,Philip E. Pfeffer,David D. Douds,E. Piotrowski,Peter J. Lammers,Yair Shachar-Hill +6 more
TL;DR: Arginine (Arg) was the predominant free AA in the ERM, and almost all Arg molecules became labeled within 3 wk of supplying (15)NH(4) (+) to the fungal compartment, which is the most likely form of N transferred to host cells following its generation from Arg breakdown.
Journal ArticleDOI
GintAMT1 encodes a functional high-affinity ammonium transporter that is expressed in the extraradical mycelium of Glomus intraradices.
Agustín López-Pedrosa,Manuel González-Guerrero,Ascensión Valderas,Concepción Azcón-Aguilar,Nuria Ferrol +4 more
TL;DR: Data suggest that GintAMT1 is involved in NH(4)(+) uptake by the extraradical mycelia from the surrounding media when it is present at micromolar concentrations, as revealed by [(14)C]methylammonium uptake experiments carried out in yeast.
Journal ArticleDOI
Role of Arbuscular Mycorrhizal Fungi in the Nitrogen Uptake of Plants: Current Knowledge and Research Gaps
Heike Bücking,Arjun Kafle +1 more
TL;DR: In this review, current knowledge about nitrogen transport through the fungal hyphae and across the mycorrhizal interface is summarized, and the regulation of these pathways and major research gaps are discussed.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection.
J.M. Phillips,D. S. Hayman +1 more
TL;DR: To improve stain penetration and clearing in whole mycorrhizal roots of onion and other host plants, and in roots infected by other fungi, the following two procedures are developed, which give deeply stained fungal structures which show distinctly against the outlines of the cells in the cortex of intact roots.
Book
Sand and Water Culture Methods Used in the Study of Plant Nutrition
Abstract: Since its appearanc e in 1952, Technical Communication no. 22 of the Commonwealt h Bureau of Horticulture and Plantation Crops has come to be recognized, at least in the English language, as the standar d reference for those interested in the developmen t of sand and water culture techniques used in plant nutrition research throughout the British Commonwealth , parts of Europe, and the United States. The Second Edition of this valuabl e work wil l strengthen even further the unique position it occupies. Most (395 of its 477 pages of text) of the book is devoted to a general review of the subject . A second part deal s in somewhat more detai l with the procedures used by the author at Long Ashton. The completenes s of treatment of the subject matter is best attested by the number of references . Their listing, with complet e titles and cross-indexing , requires 55 pages, which gives an estimated number of 1,800 references . The earliest reference date noted was 1699, the latest , 1964. They are primarily from Great Britain and the United States, but paper s from German, French, Russian, and Japanes e publications are also included. The section devoted to the historical developmen t of plant culture techniques is much longer than in the First Edition. The book represent s very much more than a historical account ing, however . It provides in great detail, and with numerous drawings where appropriate, information on every aspect of plant nutrition research . Likely problems that might be encountere d are presented , and the advantage s and disadvantage s of many of the procedures employed and material s used are discussed . A very useful feature of the book are the conclusions and summaries given after some of the topics are discusse d in detail. It wil l serve well the needs of both the researche r actively engaged in plant nutrition studies as well as the teacher seeking material for classroom presentation . With one important exception, the headings into which the subject matter is divided are identical to those of the earlier edition. However, many sections have been completel y revised, and the more recent findings have been added to practicall y all of them. A 30-page section on the "Assessmen t of contamination and the limitations of micronutrient deficiency studies" is a new and valuable addition to the Second Edition. Much of the material which was previousl y reviewed by R. E. Thiers and R. L. Mitchell is included in this section, but the results of some of the studies at Long Ashton are also presented . In the reviewer' s opinion two features of the book could be improved. I found that it took some time to get accustome d to the numbering system used for chapters , sections, and subsections , and the designation of topics in the index in this manner.Search iri g for topics in the text designate d by this numbering system is much slower than finding them by page number designation. A second shortcoming of the book is the kind of binding used. Unless the copy I received was not typical, the pages are not bound securel y and consequentl y wil l pull loose quite readily. I hope this is not generall y true as I believe that the book wil l be subject to heavy use in any laborator y where plant nutrition studies are being conducted.—V. V. Rendig, Dept, of Soils & Plant Nutrition, University of California, Dans.
Journal ArticleDOI
Early events of vesicular-arbuscular mycorrhiza formation on Ri T-DNA transformed roots
G. Bécard,Josée Fortin +1 more
TL;DR: An in vitro system using Ri T-DXA transformed roots and the vesicular-arbuscularmycorrhizal fungus Gigaspora margarita Becker & Hall has been developed to study the initial events of mycorrhiza formation.