Journal ArticleDOI
Diffusion of autoinducer is involved in regulation of the Vibrio fischeri luminescence system.
Heidi B. Kaplan,E. P. Greenberg +1 more
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A model for autoinduction is described wherein autoinducer association with cells is by simple diffusion and binding ofautoinducer to its active site is reversible.Abstract:
The enzymes for luminescence in Vibrio fischeri are induced by the accumulation of a species-specific metabolite (autoinducer) in the culture medium Tritium-labeled autoinducer was used to study the mechanism of autoinduction When 3H-autoinducer was added to suspensions of V fischeri or Escherichia coli, cellular concentrations equaled external concentrations For V fischeri, equilibration of 3H-autoinducer was rapid (within 20 s), and greater than 90% of the cellular tritium remained in unmodified autoinducer When V fischeri or E coli cells containing 3H-autoinducer were transferred to autoinducer-free buffer, 85 to 995% of the radiotracer escaped from the cells, depending on the strain Concentrations of autoinducer as low as 10 nM, which is equivalent to 1 or 2 molecules per cell, were sufficient for induction, and the maximal response to autoinducer occurred at about 200 nM If external autoinducer concentrations were decreased to below 10 nM after induction had commenced, the induction response did not continue Based on this study, a model for autoinduction is described wherein autoinducer association with cells is by simple diffusion and binding of autoinducer to its active site is reversibleread more
Citations
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Journal ArticleDOI
Quorum Sensing in Bacteria
TL;DR: The evolution of quorum sensing systems in bacteria could, therefore, have been one of the early steps in the development of multicellularity.
Journal ArticleDOI
QUORUM SENSING: Cell-to-Cell Communication in Bacteria
TL;DR: This review focuses on the architectures of bacterial chemical communication networks; how chemical information is integrated, processed, and transduced to control gene expression; how intra- and interspecies cell-cell communication is accomplished; and the intriguing possibility of prokaryote-eukaryote cross-communication.
Journal ArticleDOI
Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators.
TL;DR: How the marine luminescent bacterium V. fischeri uses the LuxR and LuxI proteins for intercellular communication is reviewed and a newly discovered family of LuxRand LuxI homologs in diverse bacterial species is described.
Journal ArticleDOI
Bacterial Quorum Sensing: Its Role in Virulence and Possibilities for Its Control
TL;DR: This work reviews the quorum-sensing circuits of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Vibrio cholerae and examines recent efforts to inhibit quorum sensing in these pathogens with the goal of designing novel antimicrobial therapeutics.
Journal ArticleDOI
Quorum‐sensing in Gram‐negative bacteria
Neil A. Whitehead,Anne M. L. Barnard,Holly Slater,Natalie J. L. Simpson,George P. C. Salmond +4 more
TL;DR: The current state of research concerning acyl H SL-mediated quorum-sensing is reviewed and two non-acyl HSL-based systems utilised by the phytopathogens Ralstonia solanacearum and Xanthomonas campestris are described.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
UseofNuclepore Filters forCounting Bacteria by Fluorescence Microscopy
TL;DR: Polycarbonate Nuclepore filters are better than cellulose filters for the direct counting of bacteria because they have uniform pore size and a flat surface that retains all of the bacteria on top of the filter.
Journal ArticleDOI
Use of nuclepore filters for counting bacteria by fluorescence microscopy.
TL;DR: Polycarbonate Nuclepore filters are better than cellulose filters for the direct counting of bacteria because they have uniform pore size and a flat surface that retains all of the bacteria on top of the filter.
Journal ArticleDOI
Cellular Control of the Synthesis and Activity of the Bacterial Luminescent System
Kenneth H. Nealson,Kenneth H. Nealson,Terry Platt,Terry Platt,J. Woodland Hastings,J. Woodland Hastings +5 more
TL;DR: In bioluminescent bacteria growing in shake flasks, the enzyme luciferase has been shown to be synthesized in a relatively short burst during the period of exponential growth, attributed to a stimulation of existing patterns of synthesis.