Journal ArticleDOI
Direct measurement of DNA unwinding angle in specific interaction between lac operator and repressor.
R. Kim,Sung-Hou Kim +1 more
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This article is published in Cold Spring Harbor Symposia on Quantitative Biology.The article was published on 1983-01-01. It has received 21 citations till now. The article focuses on the topics: lac operon & Repressor.read more
Citations
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Journal ArticleDOI
Crystal structure of the lactose operon repressor and its complexes with DNA and inducer.
Mitchell Lewis,Geoffrey Chang,Nancy C. Horton,M. A. Kercher,H. C. Pace,Maria A. Schumacher,Richard G. Brennan,Ponzy Lu +7 more
TL;DR: In this article, the three-dimensional structures of the intact lac repressor, the Lac repressor bound to the gratuitous inducer isopropyl-β-D-1-thiogalactoside (IPTG), and the lac Repressor complexed with a 21-base pair symmetric operator DNA have been determined.
Journal ArticleDOI
A perfectly symmetric lac operator binds the lac repressor very tightly.
TL;DR: A completely symmetric DNA segment has been constructed that binds the lactose repressor of Escherichia coli 10-fold more tightly than does the natural lactose operator sequence, supporting the idea that the tetrameric repressor is designed for a two-module binding to DNA.
Journal ArticleDOI
Allosteric underwinding of DNA is a critical step in positive control of transcription by Hg-MerR.
TL;DR: The magnitude and the direction of the Hg–MerR-induced change in twist angle are consistent with a positive control mechanism involving reorientation of conserved, but suboptimally phased, promoter elements and are inconsistent with a role for torsional stress in formation of an open complex.
Journal ArticleDOI
DNA dynamic flexibility and protein recognition: Differential stimulation by bacterial histone-like protein HU
Yehuda Flashner,Jay D. Gralla +1 more
TL;DR: The abundant E. coli "histone-like" protein HU is shown to be a differential effector of DNA recognition by three diverse control proteins, suggesting that the various DNA bends and distortions induced during assembly of higher order HU:DNA structures are important for the HU stimulation.
Journal ArticleDOI
Bending and twisting dynamics of short linear DNAs. Analysis of the triplet anisotropy decay of a 209 base pair fragment by Brownian simulation
TL;DR: Brownian dynamics is used to simulate the decay anisotropy of short linear DNA fragments modeled as a string of beads as mentioned in this paper, which is sufficiently general to allow for static bends, anisotropic bending, and elastic constants for bending and twisting which can vary along the chain.
References
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DNA-Protein Interactions
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