Diversity and structure of landraces of Agave grown for spirits under traditional agriculture: A comparison with wild populations of A. angustifolia (Agavaceae) and commercial plantations of A. tequilana

TLDR: Using intersimple sequence repeats and Bayesian estimators of diversity and structure, it is found that A. angustifolia traditional landraces had a genetic diversity similar to its wild populations and a higher genetic structure than the blue agave commercial system.

Abstract: Traditional farming communities frequently maintain high levels of agrobiodiversity, so understanding their agricultural practices is a priority for biodiversity conservation. The cultural origin of agave spirits (mezcals) from west-central Mexico is in the southern part of the state of Jalisco where traditional farmers cultivate more than 20 landraces of Agave angustifolia Haw. in agroecosystems that include in situ management of wild populations. These systems, rooted in a 9000-year-old tradition of using agaves as food in Mesoamerica, are endangered by the expansion of commercial monoculture plantations of the blue agave variety ( A. tequilana Weber var. Azul), the only agave certifi ed for sale as tequila, the best-known mezcal. Using intersimple sequence repeats and Bayesian estimators of diversity and structure, we found that A. angustifolia traditional landraces had a genetic diversity ( H BT = 0.442) similar to its wild populations ( H BT = 0.428) and a higher genetic structure ( θ B = 0.405; θ B =0. 212). In contrast, the genetic diversity in the blue agave commercial system ( H B = 0.118) was 73% lower. Changes to agave spirits certifi cation laws to allow the conservation of current genetic, ecological and cultural diversity can play a key role in the preservation of the traditional agroecosystems.