Patent
DNA sequence for expressing 3 alpha-hydroxysteroid dehydrogenase in Pseudomonas aeruginosa
TLDR
In this article, a DNA sequence of a polypeptide chain with 3 alpha-HSD (3 alpha-hydroxysteroid dehydrogenase) activity was revealed.Abstract:
The invention discloses a DNA sequence of a polypeptide chain with 3 alpha-HSD (3 alpha-hydroxysteroid dehydrogenase) activity The DNA sequence comprises a complementary chain sequence The DNA sequence is characterized in that an expression product of the DNA sequence has 3 alpha-hydroxysteroid dehydrogenase activity and is a novel Pseudomonas aeruginosa 3 alpha-hydroxysteroid dehydrogenase The DNA sequence comprises the following contents: a pseudomonas aeruginosa 3 alpha-hydroxysteriod dehydrogenase gene sequence obtained by utilizing bioinformatics analysis, a recombinant expression vector which contains the pseudomonas aeruginosa 3 alpha-hydroxysteroid dehydrogenase gene sequence, a genetically engineered bacterium which expresses the 3 alpha-hydroxysteroid dehydrogenase 3-hsdA and an enzyme kinetics parameter of the 3 alpha-hydroxysteroid dehydrogenaseread more
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Patent
Expression vector for enhancing gene expression of 3 beta-HSD, construction method and application thereof
TL;DR: In this article, an expression vector for enhancing gene expression of 3 beta-HSD, a construction method and an application thereof is presented, which can be transfected into human cells, and expresses 3 beta -HSD genes in the human cells in a continuous-stable-efficient manner, also can improve the damage action of a plasticizing agent on tumor cells and is expected to application in anti-tumor drugs.
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Journal ArticleDOI
Pseudomonas aeruginosa Genome Database and PseudoCAP: facilitating community-based, continually updated, genome annotation.
Geoffrey L. Winsor,Raymond Lo,Shannan J. Ho Sui,Korine S.E. Ung,Shaoshan Huang,Dean Cheng,Wai Kay Ho Ching,Robert E. W. Hancock,Fiona S. L. Brinkman +8 more
TL;DR: This database system, with its focus on facilitating flexible queries of the data and providing access to both peer-reviewed annotations as well as alternate annotation information, may be a suitable model for other genome projects wishing to use a continually updated, community-based annotation approach.
Journal ArticleDOI
ClpXP proteases positively regulate alginate overexpression and mucoid conversion in Pseudomonas aeruginosa
TL;DR: It is reported that a mutation in a stable mucoid strain derived from the parental strain PAO1, designated PAO581, that does not contain the mucA22 allele was due to a single-base deletion in mucA (DeltaT180), generating another type of C-terminal truncation.
Patent
Method for producing D-alpha-hydroxybutyric acid
TL;DR: In this article, a method for producing D-alpha-hydroxybutyric acid was proposed, which comprises the following steps: (1) preparing complete cell suspension containing NAD-independent hydroxy acid dehydrogenase or coarse enzyme liquid thereof with pseudomonas; (2) carrying out thermotropy on the complete cell suspensions or the coarse enzymes liquid thereof prepared by the thermal denaturation step.
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