Journal ArticleDOI
Dynamic behavior of liquid droplets with enzyme compartmentalization triggered by sequential glycolytic enzyme reactions.
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TLDR
In this article, a model LLPS system coupled with a sequential glycolytic enzymatic reaction was developed to reproduce the dynamic control of liquid droplets; the droplets, which consist of poly-L-lysine and nucleotides, compartmentalize two different enzymes (hexokinase and glucose-6-phosphate dehydrogenase) individually, accelerating the overall reaction.About:
This article is published in Chemical Communications.The article was published on 2021-11-23. It has received 6 citations till now.read more
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Journal ArticleDOI
Complex coacervation and compartmentalized conversion of prebiotically relevant metabolites
TL;DR: In this article , a wide variety of small anionic metabolites can form complex coacervate protocells with oligoarginine (R10) by phase separation, and they reveal the intricate interplay between prebiotically relevant molecules that combine these elements can provide insight into the requirements for the formation of life-like protocells from abiotic building blocks.
Journal ArticleDOI
Phase-Separation Propensity of Non-ionic Amino Acids in Peptide-Based Complex Coacervation Systems.
Yuto Akahoshi,Hiroka Sugai,Masahiro Mimura,Yoichi Shinkai,Ryoji Kurita,Kentaro Shiraki,Shunsuke Tomita +6 more
TL;DR: The approach to quantify the contribution of non-ionic amino acids can be expected to help to provide a more accurate description and prediction of the LLPS propensity of peptides/proteins.
Posted ContentDOI
De novo peptides that induce the liquid-liquid phase separation of α-synuclein
TL;DR: In this article , target-specific peptides are used to control liquid-liquid phase separation (LLPS) of proteins and modulate the physical nature of condensate, which can be used to gain deeper insights in the LLPS-mediated amyloid formation.
Journal ArticleDOI
Quantification of the concentration in a droplet formed by liquid–liquid phase separation of G-quadruplex-forming RNA
Kohei Yokosawa,Mitsuki Tsuruta,Shinji Kajimoto,Naoki Sugimoto,Daisuke Miyoshi,Takakazu Nakabayashi +5 more
TL;DR: In this article , the concentration of a G-quadruplex-forming RNA and an RGG peptide in a single droplet using Raman microscopy was found to maintain even when the introduced concentration ratio of these two species was varied.
Journal ArticleDOI
The roles of molecular chaperones in regulating cell metabolism
TL;DR: In this article , the Hsp90 and Hsp70 chaperone families act on human metabolic enzymes and their supramolecular assemblies to change enzymatic activities and metabolite flux.
References
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Journal ArticleDOI
RNA catalysis through compartmentalization
Christopher A. Strulson,Rosalynn C. Molden,Rosalynn C. Molden,Christine D. Keating,Philip C. Bevilacqua +4 more
TL;DR: It is shown that the concentration of RNA is enriched by up to 3,000-fold in the dextran-rich phase of a polyethylene glycol/dextran ATPS and this can lead to approximately 70-fold increase in the rate of ribozyme cleavage.
Journal ArticleDOI
Controllable protein phase separation and modular recruitment to form responsive membraneless organelles.
Benjamin S. Schuster,Ellen H. Reed,Ranganath Parthasarathy,Craig N. Jahnke,Reese M. Caldwell,Jessica G. Bermudez,Holly Ramage,Matthew C. Good,Daniel A. Hammer +8 more
TL;DR: A single-protein-based platform for generating synthetic membraneless compartments that is capable of enzymatically-triggered alterations to phase behavior and of recruiting and concentrating cargo proteins is presented.
Journal ArticleDOI
Spatial organization of enzymes for metabolic engineering.
TL;DR: A critical challenge for achieving ideal pathway compartmentalization with protein shells will likely be evolving pores to selectively limit intermediate diffusion, which should enhance the ability to rationally design metabolic pathways.
Journal ArticleDOI
Rational design of functional and tunable oscillating enzymatic networks
Sergey Semenov,Albert S. Y. Wong,van der R M Made,Sgj Postma,Joost Groen,van Hwh Rik Roekel,de Tfa Tom Greef,Wilhelm T. S. Huck +7 more
TL;DR: It is demonstrated that a network built around autoactivation and delayed negative feedback of the enzyme trypsin is capable of producing sustained oscillating concentrations of activetrypsin for over 65 h.
Journal ArticleDOI
On the origin of intracellular compartmentation and organized metabolic systems.
TL;DR: The ideas of compartmentation came into existence from the necessity to explain important physiological phenomena, in particular in heart research and in cardiac electrophysiology, which demonstrated the physiological importance of the biophysical and biochemical mechanisms described in this review.