Journal ArticleDOI
Effect of actinomycin D on early axial development in chick embryos.
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TLDR
The differential response of the embryonic axis to actinomycin D is considered to be based on a greater demand for cells in the unsegmented and nodal region, and reduced availability of cells for growth and development.Abstract:
A study was made of the uptake of actinomycin D, 3H-thymidine, and 3H-uridine in the axial regions of 11- to 13-somite chick embryos cultured in vitro. Actinomycin D was taken up into all regions of embryos, with significantly more actinomycin D per cell in the axial region, including the node and unsegmented axial mesoderm (the lower axis). Demonstrable effects on RNA synthesis were restricted to tissue in the lower axial region. However, inhibition of DNA synthesis was seen in tissues of the upper axis already segmented, as well as in the lower axis. The differential response of the embryonic axis to actinomycin D is considered to be based on a greater demand for cells in the unsegmented and nodal region. Actinomycin D inhibited RNA synthesis, thereby inhibited DNA synthesis and cell division, and reduced availability of cells for growth and development. The paucity of cells in the node and unsegmented axial mesoderm restricted the formation of new somites and the laying down of new nerve cord and notochordal tissue. The upper axial region was not permanently affected.read more
Citations
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Cell Death and Reduced Proliferative Rate
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References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Journal ArticleDOI
A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues.
TL;DR: The methods used at present for the determination of the higher nucleic acids in organs are based on certain color reactions of their carbohydrate components, but if the quantitative estimations of desoxyribonucleic acid and of ribon nucleic acid could be based on phosphorus determinations rather than on color tests of their carbohydrates components, these difficulties might be avoided.
Journal ArticleDOI
An Improved Diphenylamine Method for the Estimation of Deoxyribonucleic Acid
K. W. Giles,A. Myers +1 more
TL;DR: In this article, the Burton diphenylamine method was used to estimate extreme sensitivity coupled with a low reagent blank, which was simplified and the blank considerably reduced and the sensitivity increased.
Book ChapterDOI
[99] Determination of nucleic acids in tissues by pentose analysis
TL;DR: The extraction methods described were developed for nucleic acid determinations by spectrophotometric methods and it has become quite clear that the separations are not sufficiently refined for isotopic work.
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