Effect of dihydroergotamine and growth hormone on lipolysis in isolated fat cells of the rat

TLDR: Dihydroergotamine (DHE) islipolytic in the isolated fat cell preparation of Rodbell and the augmented lipolytic effect is suppressible with puromycin.

Abstract: Dihydroergotamine (DHE) is lipolytic in the isolated fat cell preparation of Rodbell. Bovine growth hormone (GH) alone or in combination with dexamethasone causes lipolysis that is barely measurable. The combination of GH and DHE is equally ineffective. Incubation of fat cells with all three agents produces massive lipolysis that is by far greater than the sum of effects of individual components. The augmented lipolytic effect is suppressible with puromycin. The exact mechanism of interaction between GH, dexamethasone, and DHE has to be elucidated.