Effects of sodium chloride on steady-state growth and metabolism of Saccharomyces cerevisiae.
TLDR
Sodium chloride decreased the maximum specific growth rate of Saccharomyces cerevisiae and Chemostat experiments showed this to be largely due to an increased requirement for energy-yielding substrate, apparently linked to maintenance and leading to a decrease in the yield.Abstract:
SUMMARY: Sodium chloride decreased the maximum specific growth rate of Saccharomyces cerevisiae Chemostat experiments showed this to be largely due to an increased requirement for energy-yielding substrate, apparently linked to maintenance and leading to a decrease in the yield The increased maintenance requirement is probably concerned with maintaining an intracellular Na+ concentration ten times lower than the extracellular concentration NaCl caused much higher concentrations of glucose to be required to maintain any particular glucose-uptake rate; it also increased the production of glycerolread more
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Journal ArticleDOI
Rewriting yeast central carbon metabolism for industrial isoprenoid production
Adam L. Meadows,Kristy Michelle Hawkins,Yoseph Tsegaye,Eugene Antipov,Youngnyun Kim,Lauren Raetz,Robert H. Dahl,Anna Tai,Tina Mahatdejkul-Meadows,Lan Xu,Lishan Zhao,Madhukar S. Dasika,Abhishek Murarka,Jacob R. Lenihan,Diana Eng,Joshua S. Leng,Chi-Li Liu,Jared W. Wenger,Hanxiao Jiang,Lily Chao,Patrick J. Westfall,Jefferson Lai,Savita Ganesan,Peter K. Jackson,Robert Mans,Darren Platt,Christopher D. Reeves,Poonam R. Saija,Gale Wichmann,Victor F. Holmes,Kirsten R. Benjamin,Paul W. Hill,Timothy S. Gardner,Annie Ening Tsong +33 more
TL;DR: Four non-native metabolic reactions are used to rewire central carbon metabolism in S. cerevisiae, enabling biosynthesis of cytosolic acetyl coenzyme A (acetyl-CoA, the two-carbon isoprenoid precursor) with a reduced ATP requirement, reduced loss of carbon to CO2-emitting reactions, and improved pathway redox balance.
Book ChapterDOI
Physiology of osmotolerance in fungi
Anders Blomberg,Lennart Adler +1 more
TL;DR: Combined genetic and physiological analysis is required for a deeper understanding of fungus-water relations and has revealed sequential induction of osmotically controlled genes in enteric bacteria and given exciting insights in signal transduction and regulation of the process.
Journal ArticleDOI
High‐level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by Saccharomyces cerevisiae?
Marko Kuyper,Harry R. Harhangi,Ann Kristin Stave,Aaron Adriaan Winkler,Mike S. M. Jetten,Wim T. A. M. de Laat,Jan J J den Ridder,Huub J. M. Op den Camp,Johannes P. van Dijken,Jack T. Pronk +9 more
TL;DR: In this article, it was shown that xylose metabolism in the anaerobic cellulolytic fungus Piromyces sp. E2 proceeds via a xyloses isomerase rather than via the xylos reductase/xylitol-dehydrogenase pathway found in xylosity-metabolising yeasts.
References
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Journal ArticleDOI
The Maintenance Energy of Bacteria in Growing Cultures
TL;DR: From the laws of growth, a simple relation between the maintenance requirement, the growth yield and the growth rate is derived and is shown to be in good agreement with the available data.
Journal ArticleDOI
Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition
TL;DR: It is concluded that glucose transport was the rate-limiting step of anaerobic fermentation of S. cerevisiae and of growth of the mutant and that l-sorbose is a competitive inhibitor of active glucose transport in this yeast.