Journal ArticleDOI
Electrospray ionization for mass spectrometry of large biomolecules
TLDR
Spectra have been obtained for biopolymers including oligonucleotides and proteins, the latter having molecular weights up to 130,000, with as yet no evidence of an upper limit.Abstract:
Electrospray ionization has recently emerged as a powerful technique for producing intact ions in vacuo from large and complex species in solution. To an extent greater than has previously been possible with the more familiar "soft" ionization methods, this technique makes the power and elegance of mass spectrometric analysis applicable to the large and fragile polar molecules that play such vital roles in biological systems. The distinguishing features of electrospray spectra for large molecules are coherent sequences of peaks whose component ions are multiply charged, the ions of each peak differing by one charge from those of adjacent neighbors in the sequence. Spectra have been obtained for biopolymers including oligonucleotides and proteins, the latter having molecular weights up to 130,000, with as yet no evidence of an upper limit.read more
Citations
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Journal ArticleDOI
Mass spectrometry-based proteomics
Ruedi Aebersold,Matthias Mann +1 more
TL;DR: The ability of mass spectrometry to identify and, increasingly, to precisely quantify thousands of proteins from complex samples can be expected to impact broadly on biology and medicine.
Journal ArticleDOI
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.
TL;DR: The approach described in this manuscript provides a convenient method to interpret tandem mass spectra with known sequences in a protein database.
Journal ArticleDOI
FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex.
Marta Muzio,Arul M. Chinnaiyan,Frank C. Kischkel,Karen O'Rourke,Andrej Shevchenko,Jian Ni,Carsten Scaffidi,James D. Bretz,Mei Zhang,Reiner L. Gentz,Matthias Mann,Peter H. Krammer,Marcus E. Peter,Vishva M. Dixit +13 more
TL;DR: This work utilized nano-electrospray tandem mass spectrometry to identify CAP3 and CAP4, components of the CD95 (Fas/APO-1) death-inducing signaling complex, and found a novel 55 kDa protein, designated FLICE, which has homology to both FADD and the ICE/CED-3 family of cysteine proteases.
Journal ArticleDOI
Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization
TL;DR: The DESI phenomenon was observed both in the case of conductive and insulator surfaces and for compounds ranging from nonpolar small molecules such as lycopene, the alkaloid coniceine, and small drugs, through polar compounds such as peptides and proteins.
Journal ArticleDOI
Mass spectrometry and protein analysis.
TL;DR: Recent advances in mass spectrometry instrumentation are reviewed in the context of current and emerging research strategies in protein science.
References
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Journal ArticleDOI
Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons
Michael Karas,Franz Hillenkamp +1 more
TL;DR: In the following, the first results on ultraviolet laser desorption (UVLD) of bioorganic compounds in the mass range above 10000 daltons are reported.
Journal ArticleDOI
Electrospray ion source: another variation on the free-jet theme
Masamichi Yamashita,John B. Fenn +1 more
TL;DR: In this paper, a small capillary at 2-10 kV relative to ground is electrosprayed into a bath gas to form a gaseous dispersion of ions that is expanded into vacuum in a small supersonic free jet.
Journal ArticleDOI
Molecular Beams of Macroions
TL;DR: In this article, the authors demonstrate that definite mass/charge states can be formed by electrospraying a dilute polymer solution into an evaporation chamber, negative macroions can be produced and a molecular beam formed by sampling the gaseous mixture of macroions, solvent and nitrogen molecules with a nozzle-skimmer system of the Kantrowitz-Gray type.
Journal ArticleDOI
Electrospray interface for liquid chromatographs and mass spectrometers.
TL;DR: L'effluent de chromatographie en phase liquide projete electrostatiquement dans un bain de gaz froid cree une dispersion de gouttelettes chargees qui s'evapore rapidement.
Journal ArticleDOI
On the evaporation of small ions from charged droplets
J. V. Iribarne,B. A. Thomson +1 more
TL;DR: In this paper, a theoretical argument is developed to find in which conditions this ion evaporation can occur; to that end solvation free enthalpies of cluster ions are derived from existing experimental data and the absolute reaction rate theory is applied.
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