High resolution two-dimensional electrophoresis of proteins.
TLDR
This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-05-25 and is currently open access. It has received 18633 citations till now. The article focuses on the topics: Isoelectric focusing & Free-flow electrophoresis.read more
Citations
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“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
TL;DR: The detection of murine leukemia virus antigens in complex cellular lysates was used to demonstrate the efficacy of this electrophoretic transfer technique.
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Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics.
Shao En Ong,Blagoy Blagoev,Irina Kratchmarova,Dan B. Kristensen,Hanno Steen,Akhilesh Pandey,Matthias Mann +6 more
TL;DR: SILAC is a simple, inexpensive, and accurate procedure that can be used as a quantitative proteomic approach in any cell culture system and is applied to the relative quantitation of changes in protein expression during the process of muscle cell differentiation.
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Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction
Peng Liang,Arthur B. Pardee +1 more
TL;DR: A method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction using a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer.
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The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells
TL;DR: During cell transformation and tumor devel- opment this cell type specificity of intermediate filaments is largely conserved’ and classification of tumors by their specific type of intermediate Filaments has re- cently become very valuable in clinical histodiagnosis.
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Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
TL;DR: A new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatment of fixed gels, and formation of soluble silver complexes which prevents unspecific background staining during image development.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
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Disc electrophoresis-i background and theory*
TL;DR: Some mechanisms that provide a rationale for the resolution afforded by zone electrophoresis in many gels will be detailed; the theory of some new modifications of zone electophoresis that have been designed to take maximum advantage of these mechanisms will be developed.
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Analysis of bacteriophage T7 early RNAs and proteins on slab gels
TL;DR: The RNAs and proteins specified by five early genes of bacteriophage T7 have been identified by electrophoresis on sodium dodecyl sulfate, polyacrylamide gels using a slab gel system in which 25 samples can be run simultaneously and then dried for autoradiography.
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Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
E. Kaltschmidt,H.G. Wittmann +1 more
TL;DR: A two-dimensional polyacrylamide gel system has been developed, improving the analytical separation of complex protein mixtures as obtained from ribosomes, and facilitates and accelerates considerably a number of investigations on the structure of ribosome.