Environmental Conditions During Wheat Grain Development Alter Temporal Regulation of Major Gluten Protein Genes

TLDR: The accumulation of transcripts for the major gluten storage proteins was assessed during grain development in wheat plants (Triticum aestivum L. "Butte 86" grown under seven controlled environments where temperature, water, and fertilizer conditions were varied after anthesis as mentioned in this paper.

Abstract: The accumulation of transcripts for the major gluten storage proteins was assessed during grain development in wheat plants (Triticum aestivum L. ‘Butte 86’) grown under seven controlled environments where temperature, water, and fertilizer conditions were varied after anthesis. Transcripts within the α-, γ- and ω-gliadin, low molecular weight glutenin subunit (LMW-GS), and high molecular weight glutenin subunit (HMW-GS) gene families followed similar patterns of accumulation throughout grain development under each environmental regimen. Under moderate daytime temperatures in plants that were well watered and fertilized, transcripts from all gene families were detectable by eight days postanthesis (8 DPA), were present at high levels until ≈34 DPA, and disappeared between 36 and 38 DPA. Under high temperature regimens, transcripts from all gene families appeared slightly earlier, but the time frame of accumulation was shorter. The presence or absence of postanthesis fertilizer did not alter the t...