Journal ArticleDOI
Enzyme-Carrying Polymeric Nanofibers Prepared via Electrospinning for Use as Unique Biocatalysts
Hongfei Jia,Guangyu Zhu,Bradley Vugrinovich,Woraphon Kataphinan,Darrell H. Reneker,Ping Wang +5 more
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TLDR
It appeared that the covalent binding improved the enzyme's stability against structural denaturation, such that the half‐life of the nanofibrous enzyme in methanol was 18‐fold longer than that of the native enzyme.Abstract:
Improvement of catalytic efficiency of immobilized enzymes via materials engineering was demonstrated through the preparation of bioactive nanofibers Bioactive polystyrene (PS) nanofibers with a typical diameter of 120 nm were prepared and examined for catalytic efficiency for biotransformations The nanofibers were produced by electrospinning functionalized PS, followed by the chemical attachment of a model enzyme, alpha-chymotrypsin The observed enzyme loading as determined by active site titration was up to 14% (wt/wt), corresponding to over 274% monolayer coverage of the external surface of nanofibers The apparent hydrolytic activity of the nanofibrous enzyme in aqueous solutions was over 65% of that of the native enzyme, indicating a high catalytic efficiency as compared to other forms of immobilized enzymes Furthermore, nanofibrous alpha-chymotrypsin exhibited a much-improved nonaqueous activity that was over 3 orders of magnitude higher than that of its native counterpart suspended in organic solvents including hexane and isooctane It appeared that the covalent binding also improved the enzyme's stability against structural denaturation, such that the half-life of the nanofibrous enzyme in methanol was 18-fold longer than that of the native enzymeread more
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A review on polymer nanofibers by electrospinning and their applications in nanocomposites
TL;DR: In this article, a comprehensive review is presented on the researches and developments related to electrospun polymer nanofibers including processing, structure and property characterization, applications, and modeling and simulations.
Journal ArticleDOI
Electrospinning: a fascinating fiber fabrication technique.
Nandana Bhardwaj,Subhas C. Kundu +1 more
TL;DR: This review presents an overview of the electrospinning technique with its promising advantages and potential applications, and focuses on varied applications of electrospun fibers in different fields.
Journal ArticleDOI
Electrospinning: A Fascinating Method for the Preparation of Ultrathin Fibers
TL;DR: Electrospinning is a highly versatile method to process solutions or melts, mainly of polymers, into continuous fibers with diameters ranging from a few micrometers to a few nanometers, applicable to virtually every soluble or fusible polymer.
Journal ArticleDOI
Use of electrospinning technique for biomedical applications
TL;DR: The importance of electrospinning for biomedical applications like tissue engineering drug release, wound dressing, enzyme immobilization etc. is highlighted in this paper, where the focus is also on the types of materials that have been electrospun.
Journal ArticleDOI
Electrospinning of nanofibers
TL;DR: An in-depth review of research activities on the development of nanofibers, fundamental understanding of the electrospinning process, and properties of nanostructured fibrous materials and their applications is provided in this article.
References
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Journal ArticleDOI
Nanometre diameter fibres of polymer, produced by electrospinning
Darrell H. Reneker,Iksoo Chun +1 more
TL;DR: More than 20 polymers, including polyethylene oxide, nylon, polyimide, DNA, polyaramid, and polyaniline, have been electrospun in this paper.
Journal ArticleDOI
Electrospinning process and applications of electrospun fibers
Jayesh Doshi,Darrell H. Reneker +1 more
TL;DR: In this article, the authors describe the electrospinning process, the processing conditions, fiber morphology, and some possible uses of electrospun fibers, and describe the diameter of these fibers in the range of 0.05 to 5 microns.
Journal ArticleDOI
The effect of processing variables on the morphology of electrospun nanofibers and textiles
TL;DR: In this paper, the effects of two of the most important processing parameters, spinning voltage and solution concentration, on the morphology of the fibers formed were evaluated systematically, and it was found that spinning voltage is strongly correlated with the formation of bead defects in the fibers, and that current measurements may be used to signal the onset of the processing voltage at which the bead defect density increases substantially.
Journal ArticleDOI
Bending instability of electrically charged liquid jets of polymer solutions in electrospinning
TL;DR: In this paper, the authors analyzed and explained the reasons for the instability of a viscous jet of polymer solution at a pendent droplet, showing that the longitudinal stress caused by the external electric field acting on the charge carried by the jet stabilized the straight jet for some distance.
Journal ArticleDOI
Beaded nanofibers formed during electrospinning
TL;DR: The viscoelasticity of the solution, charge density carried by the jet, and the surface tension of the polymer solution are the key factors that influence the formation of the beaded fibers as discussed by the authors.
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