Journal ArticleDOI
Evaluation of three serological tests for detection of anti-candidal antibodies in diagnosis of invasive candidiasis
TLDR
Immunoblot detection of antibody against 47 KD cytoplasmic antigen ofCandida albicans was evaluated in diagnosis of invasive candidiasis and compared to whole cell agglutination and gel diffusion tests for detection of anticandidal antibody.Abstract:
Immunoblot detection of antibody against 47 KD cytoplasmic antigen ofCandida albicans was evaluated in diagnosis of invasive candidiasis and compared to whole cell agglutination and gel diffusion tests for detection of anticandidal antibody in 64 patients. The patients included 17 with culture proved candidemia, 34 with significant candiduria (more than 10,000 colony forming units per ml of urine) and 13 with nonsignificant candiduria. Antibody against 47 KD antigen was found to be the best indicator for diagnosis of invasive candidiasis even in patients with malignancy. The sensitivity of this procedure was 82.4%, specificity 86.7%, positive predictive value 77.8%, negative predictive value 89.7% and efficacy 85.1%. The gel diffusion procedure lacked in sensitivity whereas whole cell agglutination lacked in specificity. Detection of antibody against 47 KD antigen proved to be a valuable adjunct in the diagnosis of invasive candidiasis.read more
Citations
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Journal ArticleDOI
Invasive candidiasis in the intensive care unit.
TL;DR: Invasive candidiasis is a problem associated with substantial morbidity and mortality that is highly prevalent in the intensive care unit setting and the role of newer antifungal agents for this population remains to be determined.
Journal ArticleDOI
Prophylaxis and treatment of invasive candidiasis in the intensive care setting.
TL;DR: The term “invasive candidiasis” encompasses a group of infections of increasing relevance in the intensive care setting that need to be developed to predict a high likelihood of disease so that targeted prophylaxis can be offered.
Journal ArticleDOI
Fungal colonization in patients with chronic respiratory diseases from Himalayan region of India
TL;DR: The point-prevalence of fungal colonization was almost 50%.
Journal ArticleDOI
Use of monoclonal antibody in diagnosis of candidiasis caused by Candida albicans: detection of circulating aspartyl proteinase antigen.
Byoung-Kuk Na,Chul-Yong Song +1 more
TL;DR: Results indicate that the inhibition ELISA with MAb CAP1 is effective in detection of circulating SAP antigen and that this assay may be useful for diagnosis and treatment monitoring of invasive candidiasis.
Journal ArticleDOI
Candiduria in non-neutropenic critically-ill surgical patients. Detection of IgA, IgG and IgM antibodies to Candida albicans by germ tube immunofluorescence
TL;DR: Indirect immunofluorescence with blastospores, although more sensitive than germ tube immun ofluorescence, cannot distinguish muco‐cutaneous candidosis from systemic candidosis, and is regarded a useful complementary test to evaluate candiduria in nonneutropenic, HIV‐negative, critically ill patients.
References
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An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans
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Journal ArticleDOI
Detection of circulating candida enolase by immunoassay in patients with cancer and invasive candidiasis.
Thomas J. Walsh,James W. Hathorn,Jack D. Sobel,William G. Merz,Veronica Sanchez,S. Melissa Maret,Helen R. Buckley,Michael A. Pfaller,Robert L. Schaufele,Clara A. Sliva,Eileen Navarro,J Lecciones,Pranatharthi H. Chandrasekar,James J. Lee,Philip A. Pizzo +14 more
TL;DR: Candida enolase antigenemia is a novel marker for invasive candidiasis that may be a useful indicator of deep infection in patients with cancer and neutropenia and may complement the diagnostic usefulness of blood cultures.
Journal ArticleDOI
Current trends in immunodiagnosis of candidiasis and aspergillosis.
Louis de Repentigny,Errol Reiss +1 more
TL;DR: The methods and reagents currently used for the immunodiagnosis of aspergillosis and candidiasis are reviewed and studies are being carried out to increase the sensitivity and reliability of these tests through the use of monoclonal antibodies and purified, clinically relevant antigens.