Journal ArticleDOI
Exocytosis and membrane recycling.
J. Meldolesi,B. Ceccarelli +1 more
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Direct evidence, obtained in some secretory systems, indicates that after exocytosis the granules and plasma membranes do not intermix, but remain segregated.Abstract:
Exocytosis implies the fusion of the membrane of secretion granules with, and the insertion into, the plasmalemma. In non-growing systems such an insertion is temporary in that the inserted membrane is eventually removed. Turnover results indicate that the removed membrane is not destroyed but recycled within the cell and reused. In some systems exocytosis occurs over the entire plasmalemma, while in others it is restricted to discrete regions, characterized by peculiar morphology and composition. Thus the fusion of the two membranes is probably preceded by a recognition step. Structural specializations were detected in interacting granule and plasma membranes by freeze-fracture and surface labelling techniques: arrays of intramembrane particles in protozoans and nerve terminals; clearing of particles and surface antigens in other systems. Direct evidence, obtained in some secretory systems, indicates that after exocytosis the granules and plasma membranes do not intermix, but remain segregated. The subsequent recapture of membrane patches of the granule type (in many systems by means of coated pits and vesicles) could then account for the striking specificity of the recycling process, documented by both composition and structural studies. In different systems the recycling of granule membranes is carried out at greatly different rates. Recent results in the parotid gland and neuromuscular junction indicate that this process is Ca2+-dependent.read more
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Vesicle pools and Ca2+ microdomains: new tools for understanding their roles in neurotransmitter release.
TL;DR: The results obtained allowed us to assess the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds.
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Secretory granules are recaptured largely intact after stimulated exocytosis in cultured endocrine cells
TL;DR: It is concluded that most granules reseal in <10 s after releasing cargo, and that these empty or partially empty granules are recaptured otherwise intact.
Journal ArticleDOI
Neurotransmitter release: fusion or 'kiss-and-run'?
TL;DR: Evidence is looked at that quantal release of neurotransmitter from clear synaptic vesicles may occur by a similar 'kiss-and-run' mechanism to that of exocytosis.
Journal ArticleDOI
Rapid endocytosis coupled to exocytosis in adrenal chromaffin cells involves Ca2+, GTP, and dynamin but not clathrin
TL;DR: Whereas anti-dynamin antibodies blocked RE, anti-clathrin antibodies did not, suggesting that clathrin-coated vesicles are not involved in this form of endocytosis, and RE may represent the initial step in the rapid recycling of secretory granules in the chromaffin cell.
Journal ArticleDOI
Structure and function of fusion pores in exocytosis and ectoplasmic membrane fusion
Manfred Lindau,Wolfhard Almers +1 more
TL;DR: Investigation of single fusion events by electrophysiological techniques together with fluorimetric measurements have provided some insight into the properties of the first aqueous connection, the fusion pore, indicating that it is initially not a purely lipidic structure, but incorporates lipids when it expands.
References
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Journal ArticleDOI
Intracellular aspects of the process of protein synthesis
TL;DR: The title of the Nobel Lecture of George Palade (1 August, p. 347) should have been "Intracellular aspects of the process of protein secretion."
Journal ArticleDOI
Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction
John E. Heuser,Thomas S. Reese +1 more
TL;DR: During stimulation the intracellular compartments of this synapse change shape and take up extracellular protein in a manner which indicates that synaptic vesicle membrane added to the surface during exocytosis is retrieved by coated vesicles and recycled into new synaptic vESicles by way of intermediate cisternae.
Journal ArticleDOI
Turnover of transmitter and synaptic vesicles at the frog neuromuscular junction
TL;DR: It is suggested that synaptic vesicles fuse with, and re-form from, the membrane of the nerve terminal during and after stimulation and that the re-formed vesicle can store and release transmitter.
Journal ArticleDOI
Functional changes in frog neuromuscular junctions studied with freeze-fracture.
TL;DR: In freeze-fractured frog sartorius muscles, the long terminal branches of motor axons possess a series of narrow transverse ridges on their surface, bordered by rows of relatively large particles within the presynaptic membrane, which represent anen face view of the electron-dense cytoplasmic bands around which synaptic vesicles cluster.
Journal ArticleDOI
Th molecular organization of adrenal chromaffin granules
Hans Winkler,E. Westhead +1 more
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