Experimental infection of layer hens with a human isolate of Brachyspira pilosicoli
TLDR
Findings confirm that B. pilosicoli strains can infect across species barriers and cause chronic mild diarrhoea in intact adult chickens.Abstract:
The anaerobic intestinal spirochaete Brachyspira pilosicoli commonly colonizes the large intestine of a number of species, including chickens and human beings. The purpose of the current study was to determine whether an isolate of B. pilosicoli recovered from an HIV-infected patient with diarrhoea could infect and cause disease in adult chickens. Over a 4-week period following experimental infection, a group of eight inoculated chickens showed a persistent and significant increase in faecal water content (∼6–7 %). The faeces of three of the eight birds became culture-positive, and remained so. At post-mortem examination, no specific pathological changes were found, and no spirochaetal attachment to the caecal epithelium was observed. These findings confirm that B. pilosicoli strains can infect across species barriers and cause chronic mild diarrhoea in intact adult chickens.read more
Citations
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Journal ArticleDOI
Brachyspira spp. and avian intestinal spirochaetosis: an epidemiological review
TL;DR: This review summarises the species of Brachyspira that have been associated with AIS and provides an overview of epidemiological studies that have investigated prevalence, risk factors, and preventive measures for Brachypira spp.
Dissertation
Brachyspira infection in laying hens
TL;DR: Development of a multiplex qPCR for detection and quantitation of pathogenic intestinal spirochaetes in the faeces of pigs and chickens and the role of Brachyspira in this situation is studied.
OtherDOI
Other Bacterial Diseases
Catherine M. Logue,Claire B. Andreasen,Luke B. Borst,Helena Eriksson,David J. Hampson,Susan Sanchez,Richard M. Fulton +6 more
TL;DR: Disease syndromes included in the chapter include beak necrosis, venereal disease of geese, and liver granulomas, but are not identified to a specific organism responsible because of the multifactorial nature of the disease.
Journal ArticleDOI
Brachyspira pilosicoli isolated from two beagles and one mongrel in Japan.
TL;DR: The feces, colonic contents, and colonic mucosa from two beagles and a mongrel with and without mucohemorrhagic diarrhea were anaerobically cultured and 16SrDNA sequencing results demonstrated that the canine isolates were B. pilosicoli and the base alignments registered in DDBJ.
Journal ArticleDOI
High prevalence of Brachyspira spp. in layers kept in alternative husbandry systems associated with frequent species variations from end of rearing to slaughter.
TL;DR: The longitudinal survey revealed for the first time a possible shift in the Brachyspira species in a substantial number of layer flocks during their lifetime, and organic flocks were more often positive than flocks from barn systems.
References
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Journal ArticleDOI
Serpulina pilosicoli sp. nov., the Agent of Porcine Intestinal Spirochetosis
Darren J. Trott,Thaddeus B. Stanton,Neil S. Jensen,Gerald E. Duhamel,John L. Johnson,David J. Hampson +5 more
TL;DR: DNA-DNA relative reassociation experiments in which the S1 nuclease method was used revealed that intestinal spirochete strain P43/6/78T was related to, but was genetically distinct from, both S. hyodysenteriae B78T and S. innocens B256T, and it is proposed that strain P 43/ 6/78 should be designated as the type strain of a new species, Serpulina pilosicoli.
Journal ArticleDOI
Autoclaved liquid medium for propagation of Treponema hyodysenteriae.
TL;DR: Three liquid media that differ slightly in composition but not in the method of preparation were developed for the propagation of TrePonema hyodysenteriae and Treponema innocens and supported better growth of T. hyodysteriae than did previously used liquid media.
Journal ArticleDOI
Isolation of Serpulina pilosicoli from Rectal Biopsy Specimens Showing Evidence of Intestinal Spirochetosis
TL;DR: The association between seeing spirochetes in biopsy specimens and isolating S. pilosicoli was statistically significant, clearly indicating that thisSpirochete is the agent of IS.
Journal ArticleDOI
Selective medium for the isolation of Treponema hyodysenteriae.
SR Jenkinson,CR Wingar +1 more
Journal ArticleDOI
Experimental infection of newly weaned pigs with human and porcine strains of Serpulina pilosicoli.
TL;DR: The role of S. pilosicoli in the etiology of PIS is confirmed and evidence that S. Pilosicol strains of human origin have pathogenic potential in an animal model is provided.