Expression of IL-1β and TNF-α in the myocardiumof MCMV myocarditis and its significance

TLDR: IL-1β and TNF-α may play important roles in pathogenesis of viral myocarditis and can be found in myocardium of MCMV my Carditis model.

Abstract: Objective: To study the expression of IL-1β and TNF-α in the myocardium of murine cytomegalovirus(MCMV) myocarditis and to evaluate its effect on myocardial damage of MCMV myocarditisMethods: Sixty BALB/C mice of 4 weeks were randomly divided into two groups: 36 were injected intraperitoneally with MCMV(experimental group) and 24 served as control groupImmunohistochemistry technique was used to detect IL-1β and TNF-α expression in myocardium,and myocardial lesions were observed by histopathologyResults: Focal or diffuse lesions characterized by inflammatory cells and degeneration or necrosis of myocytes in experimental group were revealed in the myocardium;Positive staining signals of IL-1β and TNF-α proteins which mainly located in infiltrating inflammatory cells and degenerative or necrotic myocytes were markedly detectableThere were no positive findings in control groupConclusion: IL-1β and TNF-α can be found in myocardium of MCMV myocarditis modelIL-1β and TNF-α may play important roles in pathogenesis of viral myocarditis