Journal ArticleDOI
Fast and Accurate Method for Measuring Photon Flux in the Range 2500–6000 Å
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TLDR
In this paper, the authors used a 1P28 photomultiplier (PM) tube plus a solution of rhodamine B in ethylene glycol (RhB solution) to measure photon flux from pulsed and steady state light sources over a wide range of wavelength and flux.Abstract:
This paper presents a technique for fast and accurate measurement of photon flux from pulsed and steady state light sources over a wide range of wavelength and flux. The technique uses a 1P28 photomultiplier (PM) tube plus a solution of rhodamine B in ethylene glycol (RhB solution). The flux to be measured is absorbed by the rhodamine solution and the resultant rhodamine fluorescence is measured by the PM tube. The solution absorbs completely all photons below 6000 A and fluoresces with constant fluorescence spectrum and fluorescence quantum efficiency for incident wavelengths from 6000 to at least 2500 A. These properties of the RhB solution result in a PM tube‐RhB solution detecting system with constant photon sensitivity from 6000 to 2500 A. A large range of flux can be measured by adjusting the distance between the fluorescent solution and the PM tube so as to maintain the tube current within limits of stable operation. An RhB detector with a given distance between the RhB solution and the PM tube pho...read more
Citations
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Journal ArticleDOI
Transverse location of the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene in model lipid bilayer membrane systems by resonance excitation energy transfer.
TL;DR: The experiments showed that energy-transfer spectroscopy can provide useful quantitative information as to the transverse location of diphenylhexatriene in homogeneous phospholipid bilayers and may also reflect lateral partitioning of donor or of both donor and acceptor into different phases in systems exhibiting phase separations.
Book ChapterDOI
Instrumentation for Fluorescence Spectroscopy
TL;DR: In this paper, the authors discuss the properties of individual components in a spectrofluorometer and those properties of samples which affect the measured parameters, and stress the acquisition of excitation and emission spectra.
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Determination of arsenic by cathodic stripping voltammetry with a hanging mercury drop electrode
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Some β-carboline derivatives as fluorescence standards
TL;DR: In this article, the standard properties of β-carboline derivatives were studied in H2SO4 aqueous solutions, and the fluorescence spectra of these five cationic species cover a region between 400-560 nm.
Journal ArticleDOI
Environment-sensitive fluorophores with benzothiadiazole and benzoselenadiazole structures as candidate components of a fluorescent polymeric thermometer.
Seiichi Uchiyama,Kohki Kimura,Chie Gota,Kohki Okabe,Kyoko Kawamoto,Noriko Inada,Toshitada Yoshihara,Seiji Tobita +7 more
TL;DR: DBThD-IA exhibits a 10-fold higher photostability in aqueous solution than the original fluorophore DBD-IA, which allowed us to create a new robust molecular nanogel thermometer for intracellular thermometry.
References
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Journal ArticleDOI
A New Sensitive Chemical Actinometer. II. Potassium Ferrioxalate as a Standard Chemical Actinometer
C. G. Hatchard,C. A. Parker +1 more
TL;DR: In this paper, a detailed study has been made of the photolysis of the acidified solutions previously recommended for chemical actinometry, and accurate values of quantum efficiency have been determined at twelve wavelengths between 254 and 578 m$\mu $, and the effect of temperature, light intensity and of photolyte composition have been investigated.
Journal ArticleDOI
Calibration of Spectrofluorimeters for Measuring Corrected Emission Spectra
TL;DR: In this paper, a rhodamine B quantum counter and a thermopile were used to calibrate a grating monochromator and photomultiplier for the measurement of corrected emission spectra from 240 to 600 mμ.
Journal ArticleDOI
Multiplier Photo-Tube Characteristics: Application to Low Light Levels*
TL;DR: In this paper, a wide range of linearity of photo-multiplier types 931-A, 1P21, 1p22, and 1P28 is shown. But the sensitivity of these tubes has not been investigated.
Journal ArticleDOI
Quantum Yield of the Ferrioxalate Actinometer
John Lee,H. H. Seliger +1 more
TL;DR: In this paper, the quantum yield of the potassium ferrioxalate actinometer at 365/6 mμ has been measured to be 1.26±0.03 relative to a calibrated thermopile.
Journal ArticleDOI
Heterochromatic Photometry of the Ultra-Violet Region
TL;DR: In this article, it has been shown that by using fluorescent substances in conjunction with a photoelectric cell it can be made non-selective while retaining a great advantage of sensitivity.