Journal ArticleDOI
Fertilizing capacity of equine spermatozoa stored for 24 hours at 5 or 20°C
TLDR
A breeding trial was conducted to evaluate the effect of in vitro storage time and temperature on fertilizing capacity of equine spermatozoa, finding that the reduction in all motility parameters tested was more dramatic in semen stored at 20°C than that stored at 5°C.About:
This article is published in Theriogenology.The article was published on 1989-10-01. It has received 68 citations till now. The article focuses on the topics: Sperm washing & Insemination.read more
Citations
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Journal ArticleDOI
Advances in cooled semen technology
Florence Batellier,Marianne Vidament,J. Fauquant,Guy Duchamp,G Arnaud,J. M. Yvon,Michèle Magistrini +6 more
TL;DR: Results demonstrate that semen diluted in INRA96 extender and stored at 15 degrees C can be an alternative to semen dilution in milk-based extenders and storage at 4 degrees C for "poor cooler" stallions and can be as efficient at 15degree C as at 4degree C, for preserving sperm motility and fertility.
Journal ArticleDOI
Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa.
TL;DR: The spermatozoon is a highly specified cell that has the abilities of active motility and fertilization of the ovum and the functional integrity of the sperm plasma membrane can be determined by functional tests (determination of motility, resistance against hypoosmotic media) or different staining methods.
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Recent advances in cooled-semen technology
TL;DR: After slow-cooling, semen-storage and shipping is best performed at 5 degrees C, maintaining semen motility, membrane integrity and DNA integrity for up to 40 h after collection, and shipping containers created from Styrofoam boxes provide maintenance of semen quality at low cost.
Journal ArticleDOI
Effect of seminal plasma dilution or removal on spermatozoal motion characteristics of cooled stallion semen
TL;DR: In this paper, a series of experiments that evaluated spermatozoal motion characteristics, as determined by computerized semen analysis, of extended, cooled stallion semen was conducted. And the results showed that those aliquots utilizing high dilution ratios of extender (>1:2.1, volume of semen: volume of extenders) maintained higher spermato-zoal motility and mean velocity than those using a lower dilution ratio.
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Artificial insemination and preservation of semen.
TL;DR: Artificial insemination is an effective technique for improving utilization of stallions in breeding programs as discussed by the authors, and optimal pregnancy rates are attainable when proper semen handling and in-semination procedures are used.
References
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Journal ArticleDOI
Defense strategies against hypoxia and hypothermia
TL;DR: In hypoxia-tolerant animals, metabolic arrest and stabilized membrane functions are the most effective strategies for extending tolerance to Hypoxia through a number of biochemical and physiological mechanisms.
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Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa
R.P. Amann,B.W. Pickett +1 more
TL;DR: For technical reasons, including poor pregnancy rates achieved with thawed semen from many stallions, as well as restrictions imposed by registry associations, frozen semen has not been widely used by the horse industry in North America or Western Europe.
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The roles of lipid and protein in the protection of ram spermatozoa at 5°C by egg-yolk lipoprotein
TL;DR: In this article, the importance of the protein during cold storage was discussed and it was concluded that the protein of LDF serves to solubilize the lipid and bind it to the cell membrane.
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Evidence that phospholipid protects ram spermatozoa from cold shock at a plasma membrane site
TL;DR: It is concluded that the protective effect of phospholipid against cold shock in ram spermatozoa is due to a 'loose' interaction of the lipid structures with the plasma membrane of the cells.