Journal ArticleDOI
Flow cytometry and plant protoplast cell biology
TLDR
Flow cytometry, which requires individualized or single cell preparations, is particularly adapted to studies of protoplast biology and enables measurement of several parameters in single protoplasts at a high rate of analysis.Abstract:
Flow cytometry, which requires individualized or single cell preparations, is particularly adapted to studies of protoplast biology. It has been developed at three levels: descriptions of populations, measurement of cell function, and positive car negative selection of rare protoplast types. It enables measurement of several parameters in single protoplasts at a high rate of analysis. Without protoplast purification, excellent resolution of subpopulations is still possible. The quantitative data obtained can each protoplast normally have relative units but absolute calibration of intensity scale is availableread more
Citations
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Journal ArticleDOI
Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.
Hazel M. Davey,Douglas B. Kell +1 more
TL;DR: Flow cytometry is a technique, which allows one to analyze cells rapidly and individually and permits the quantitative analysis of microbial heterogeneity, and offers many advantages over conventional measurements for both routine and more exploratory analyses of microbial properties.
Journal ArticleDOI
A cytometric exercise in plant DNA histograms, with 2C values for 70 species.
Dominique Marie,Spencer Brown +1 more
TL;DR: An extensive graphical guide to interpreting DNA histograms and their problems is given, and cytometry laboratories unfamiliar with plant sciences will find herein a guide, and references, to adapt their methods to plant material.
Journal ArticleDOI
Cell cycle phase specificity of putative cyclin-dependent kinase variants in synchronized alfalfa cells.
Zoltán Magyar,Tamás Mészáros,Pál Miskolczi,Maria Deak,Attila Fehér,Spencer Brown,Eva Kondorosi,Alekos Athanasiadis,Sándor Pongor,Metin Bilgin,László Bakó,Csaba Koncz,Dénes Dudits +12 more
TL;DR: It is demonstrated here that at least four additional cdc2 homologous genes are expressed in the tetraploid alfalfa, and the observed fluctuation of transcript levels, amounts, and activities of kinases in different cell cycle phases reflects a multilevel regulatory system during cell cycle progression in plants.
Journal Article
Application of flow cytometry for the study of plant genomes
TL;DR: A review of the field of flow cytometric analysis of plant cell nuclei and chromosomes can be found in this article, where the potential of this technology for gene isolation and mapping is discussed.
Journal ArticleDOI
Comparison of four nuclear isolation buffers for plant DNA flow cytometry.
TL;DR: Each lysis buffer tested responded to a specific problem differently and none of the buffers worked best with all species, so selection of the most appropriate buffer depending on species, tissue type and the presence of cytosolic compounds interfering with DNA staining is facilitated.
References
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Journal ArticleDOI
Rapid flow cytometric analysis of the cell cycle in intact plant tissues.
David W. Galbraith,Kristi R. Harkins,Joyce M. Maddox,Nicola M. Ayres,Dharam P. Sharma,Ebrahim Firoozabady +5 more
TL;DR: The amount of nuclear DNA in the homogenates of monocotyledonous and dicotylingonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.
Journal ArticleDOI
Subcompartments of the G1 phase of cell cycle detected by flow cytometry
TL;DR: Cellular DNA and RNA were simultaneously quantitated in individual cells of cycling and quiescent populations by flow cytometry based on differences in RNA content, two distinct subcompartments, G1A and G1B, were recognized in the G1 phase of exponentially growing cells.
Journal ArticleDOI
Flow-cytometric characterization and sorting of plant chromosomes.
A. M. M. de Laat,J. Blaas +1 more
TL;DR: Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents.
Journal ArticleDOI
Microfluorimetric quantitation of cellulose biosynthesis by plant protoplasts using Calcofluor White
TL;DR: The results indicate that the amount of fluorescence emitted following Calcofluor White treatment is a specific measurement of cell wall cellulose levels.
Journal ArticleDOI
Cytometry and flow cytometry of 4′,6‐diamidino‐2‐phenylindole (DAPI)‐stained suspensions of nuclei released from fresh and fixed tissues of plants
TL;DR: Cytometry and flow cytometry were used to study characteristics of fluorescence of the DNA-DAPI complex in nuclei released from different fresh and formaldehyde-fixed pea (Pisum sativum L. cv. Lincoln) tissues.
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A cytometric exercise in plant DNA histograms, with 2C values for 70 species.
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