Journal ArticleDOI
Flow-through respirometry applied to chamber systems: pros and cons, hints and tips.
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TLDR
Two important biomedical applications of flow-through respirometry (metabolic phenotyping and room calorimetry) are discussed in detail, and a list of suggestions aimed primarily at investigators starting out in applying flow-Through respirometry is concluded.Abstract:
Flow-through respirometry is a powerful, accurate methodology for metabolic measurement that is applicable to organisms spanning a body mass range of many orders of magnitude. Concentrating on flow-through respirometry that utilizes a chamber to contain the experimental animals, we describe the most common flow measurement and control methodologies (push, pull and stop-flow) and their associated advantages and disadvantages. Objective methods for calculating air flow rates through the chamber, based on the body mass and taxon of the experimental organism, are presented. Techniques for removing the effect of water vapor dilution, including the direct measurement of water vapor pressure and mathematical compensation for its presence, are described and evaluated, as are issues surrounding the analysis of one or both of the respiratory gases (oxygen and carbon dioxide), and issues related to the mathematical correction of wash-out phenomena (response correction). Two important biomedical applications of flow-through respirometry (metabolic phenotyping and room calorimetry) are discussed in detail, and we conclude with a list of suggestions aimed primarily at investigators starting out in applying flow-through respirometry.read more
Citations
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Journal ArticleDOI
Orexin activation counteracts decreases in nonexercise activity thermogenesis (NEAT) caused by high-fat diet
TL;DR: A change in the efficiency of energy expenditure based upon diet is suggested, such that SPA during HFD burns fewer calories compared to SPA on a standard chow diet.
Journal ArticleDOI
Assessing the development and application of the accelerometry technique for estimating energy expenditure.
TL;DR: Important issues concerning the accelerometry technique for estimating energy expenditure and ODBA are discussed; deriving ODBA, calibratingODBA, acceleration logger recording frequencies, scenarios where ODBA is less likely to be valid, and the power in recording acceleration and heart rate together are discussed.
Journal ArticleDOI
The heart rate method for estimating metabolic rate: review and recommendations.
TL;DR: This review describes the range of studies which have adopted either the quantitative or qualitative approach to estimating the MR of birds, mammals and reptiles and recommends that a quantitative approach be adopted wherever possible but that this may entail deriving a calibration relationship which is practical and applicable, rather than the most accurate possible.
Journal ArticleDOI
Integration of body temperature into the analysis of energy expenditure in the mouse
TL;DR: The high post-mortem heat conductance demonstrates that most insulation in mice is via physiological mechanisms, with little from fur or fat, and suggests that the definition of the upper limit of the thermoneutral zone should be re-considered.
Journal ArticleDOI
Omega-3 long-chain polyunsaturated fatty acids support aerial insectivore performance more than food quantity
Cornelia Wingfield Twining,J. Thomas Brenna,Peter Lawrence,J. Ryan Shipley,Troy N. Tollefson,David W. Winkler +5 more
TL;DR: It is found that fatty acid composition was more important for Tree Swallow chick performance than food quantity, and chicks preferentially retainedLCPUFA in brain and muscle when both food quantity and LCPUFA were limited.
References
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Book
Measuring Metabolic Rates: A Manual for Scientists
TL;DR: Vital topics such as manual and automated baselining, implementing multi-animal systems, common pitfalls, and the correct analysis and presentation of metabolic data are covered in enough detail to turn a respirometry neophyte into a hardened metabolic warrior, ready to take on the task of publication in peer-reviewed journals with confidence.
Journal ArticleDOI
Instantaneous Measurements of Oxygen Consumption During Pre-Flight Warm-Up and Post-Flight Cooling in Sphingid and Saturniid Moths
TL;DR: The data on metabolic scope, power requirements for flight, Q 10 and body mass are used to develop equations that predict thoracic temperature during flight for both sphingids and saturniids.
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One-step N2-dilution technique for calibrating open-circuit VO2 measuring systems
TL;DR: A simple one-step procedure that eliminates the need to calibrate the O2 analyzer or measure the flow past the animal is described for calibrating an open-flow respirometry system.
Journal ArticleDOI
Muscle efficiency and elastic storage in the flight motor of Drosophila.
TL;DR: By comparison of mechanical power measurements based on gas exchange with simultaneously measured flight kinematics in Drosophila, a method was developed for determining both the mechanical efficiency and the minimum degree of elastic storage within the flight motor.
Journal ArticleDOI
The cost of foraging by a marine predator, the Weddell seal Leptonychotes weddellii: pricing by the stroke
TL;DR: The results show that the energy expended in digestion for a free-ranging marine mammal are additive to locomotor and basal costs, and the aerobic cost of diving infree-ranging seals where cryptic behavior and remote locations prevent direct energetic measurements.