Journal ArticleDOI
Formation of Hybrid Cells by Transfer of Nuclei via Fusion of Miniprotoplasts from Cell Lines of Nitrate Reductase Deficient Tobacco
TLDR
The results demonstrate that miniprotoplasts, which are characterized by a dense cytoplasm free from large vacuoles, are favourable material for fusion experiments and that fusion using minipra toplasts is an alternative way of transferring nuclear material to both nuclear transplantation and protoplast fusion.About:
This article is published in Zeitschrift für Pflanzenphysiologie.The article was published on 1979-02-01. It has received 27 citations till now. The article focuses on the topics: Nitrate reductase & Protoplast.read more
Citations
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Book ChapterDOI
Applications of Protoplasts to the Study of Plant Cells
TL;DR: This chapter examines the major applications of plant protoplasts to the study of plant cells and places emphasis on structural studies of higher plant protoplasts especially by electron microscopic methods.
BookDOI
A genetic approach to plant biochemistry.
TL;DR: There can surely be no better argu ments for a more holistic approach to biology than the massive output of knowledge from microbial "Biochemical Genetics" and the more recent revelations from "Molecular Genetic" studies of development in Droso phila.
Journal ArticleDOI
Intergeneric transfer of cytoplasmic male sterility between Raphanus sativus (cms line) and Brassica napus through cytoplast-protoplast fusion.
T. Sakai,J. Imamura +1 more
TL;DR: Cytoplasts isolated from hypocotyl protoplasts of Raphanus sativus cv Kosena by ultracentrifugation through Percoll/mannitol discontinuous gradient were fused with iodoacetamide(IOA)-treated protoplast of Brassica napus c v Westar to show hybridization patterns similar to B. napus.
Journal ArticleDOI
Correction of nitrate reductase defect in auxotrophic plant cells through protoplast-mediated intergeneric gene transfers
TL;DR: Restoration of nitrate reductase and xanthine dehydrogenase activities confirm the presence of an active form of the molybdenum-cofactor by the expression of introduced genes of Physalis and Datura into the genome of cnx-68 Nicotiana.
References
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Journal ArticleDOI
Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase
Andreas J. Müller,Reinhard Grafe +1 more
TL;DR: Differences between the NR-negative mutants with respect to chlorate resistance suggest that chlorate inhibits cultured N tabacum cells not only via its NR-catalysed conversion to chlorite, but also by NR-independent mechanisms.
Journal ArticleDOI
The induction of aggregation and fusion of Dancus carota protoplasts by polyethylene glycol
TL;DR: Light- and electron micrographs show the strong adhesion and fusion of cell membranes and the osmotic changes in the protoplasts induced by polytehylene glycol.
Journal ArticleDOI
Somatic Hybridization of Nitrate Reductase Deficient Mutants of Nicotiana tabacum by Protoplast Fusion
TL;DR: The results demonstrate that protoplast fusion can be used for the genetic analysis of cell variants of higher plants and that nitrate reductase-deficient mutants provide efficient selective systems for hybrid cells.
Journal ArticleDOI
Enucleation of Plant Protoplasts by Cytochalasin B
TL;DR: By varying the concentration of cytochalasin B and centrifugation speed, enucleated protoplasts could be prepared fromNicotiana sylvestris, Nicotiana tabacum, Pisum sativum and Daucus carota and divided at high frequency in a suitable medium.
Journal ArticleDOI
Concanavalin A Improves the Polyethylene Glycol Method for Fusing Plant Protoplasts
TL;DR: An improved method for polyethylene glycol-induced fusion of protoplasts isolated from auxotrophic mutants of Nicotiana tabacum L. cv.