Somatic hybrid plants of potato and tomato regenerated from fused protoplasts
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TLDR
In the absence of a selection system against the potato parent, the analysis of ribulose bisphosphate carboxylase provides a convenient marker to demonstrate the hybrid nature of the plants.Abstract:
Mesophyll protoplasts of Lycopersicon esculentum Mill. var. cerasiforme (Dunal) Alef, mutant yellow green 6, Rick and protoplasts of a liquid callus culture of the dihaploid strain HH258 of Solanum tuberosum L. were prepared and many fusion products were visible after the protoplasts were incubated together first in the presence of polyethylene glycol and then with a high Ca2+ ion concentration. The protoplasts were transferred to a rich medium and the resultant calli were cultured. Some calli regenerated normal green shoots which were transferred to soil or grafted onto a tomato stock. The subunit polypeptide pattern of ribulose 1,5-bisphosphate carboxylase prepared from leaf material of four regenerated plants was analyzed by isoelectric focusing. The ribulose bisphosphate carboxylase enzyme oligomer in the four plants contained the small subunit products resulting from the expression of both tomato and potato nuclear genes proving these plants to be somatic hybrids between tomato and potato. In three of the four plants the large subunit polypeptides and hence the functional chloroplast DNA were from tomato whereas in the fourth the large subunit and therefore the chloroplast DNA was derived from potato. The plant material was insufficient to establish the chromosome numbers precisely, however counts close to 50 which is near to the expected 48 were obtained for three of the hybrids whereas in the fourth a number close to 72 was observed. In the absence of a selection system against the potato parent, the analysis of ribulose bisphosphate carboxylase provides a convenient marker to demonstrate the hybrid nature of the plants.read more
Citations
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Journal ArticleDOI
RFLP Maps Based on a Common Set of Clones Reveal Modes of Chromosomal Evolution in Potato and Tomato.
TL;DR: Linkage maps based on a common set of restriction fragment length polymorphism markers provide a basis for uniting the previously separate disciplines of tomato and potato genetics and may now be possible to test theories about homologies or orthologies of other genes, including those coding for disease resistance and stress tolerances.
Book ChapterDOI
Genetics and breeding
M. Allen Stevens,Charles M. Rick +1 more
TL;DR: Many useful materials in tomato genetics have been contributed by tomato breeders, and conversely, studies in basic tomato Genetics have permitted much progress in tomato breeding.
Journal ArticleDOI
Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids
TL;DR: In this article, the chloroplast type (N. tabacum = NT or N. knightiana = NK) in the plants was determined on the basis of the species specific EcoRI restriction pattern of the chloropslast DNA.
BookDOI
Primary and secondary metabolism of plant cell cultures
TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI
Chloroplast dna evidence for the interrelationships of tomatoes, potatoes, and pepinos (solanaceae)
TL;DR: The chloroplast DNA restriction site analysis results support the controversial, but previously suggested, treatment of Lycopersicon as congeneric with Solanum in subgenus Potatoe and suggest that Cyphomandra should be included within SolAnum.
References
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Nutritional Requirements for Growth of Vicia hajastana Cells and Protoplasts at a Very Low Population Density in Liquid Media
K. N. Kao,M. R. Michayluk +1 more
TL;DR: Vicia cells were able to grow in a mineral-salt solution supplemented with sucrose (or glucose), a few vitamins, and 2,4-dichlorophenoxyacetic acid but were not able to survive when cultured at a low population density unless the medium was supplemented with zeatin, naphthalene-1-acetic Acid, nucleic-acid bases, amino acids, other sugars, sugar alcohols, and organic acids.
Journal ArticleDOI
A method for high-frequency intergeneric fusion of plant protoplasts.
K. N. Kao,M. R. Michayluk +1 more
TL;DR: None of the protoplasts from pea and barley leaves, either with or without PEG treatment, underwent cell division during the period of observation, and the mechanism of adhesion and fusion of theprotoplasts has been discussed.
Journal ArticleDOI
Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca
TL;DR: Protoplasts of soybean and N. glauca were induced to fuse with polyethylene glycol (PEG 1540) and up to 39% of the protoplasts in the treated population were heterokaryocytes, which indicated a tendency to stick together and break into pieces in the later cell generations.
Journal ArticleDOI
Synthesis and transport of the small subunit of chloroplast ribulose bisphosphate carboxylase
Peter E. Highfield,R. John Ellis +1 more
TL;DR: It is deduced that the signal hypothesis does not apply to chloroplasts; an envelope carrier mechanism is proposed.
Journal ArticleDOI
Regeneration of whole plants from isolated mesophyll protoplasts of tobacco
TL;DR: Theoret. Leningrad un.-ta Mr. Fischereiwiss (zitiert nach Woyn~rovich 1963).
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