Journal ArticleDOI
Functional analysis of the heterologous NSP1 genes in the genetic background of simian rotavirus SA11.
TLDR
The results indicated that viral growth and genome segment reassortment with other viruses may not be influenced by the presence of heterologous NSP1 and its expression level, while genomic diversity of N SP1 genes might have been associated with the relative adaptability to the genetic background of SAff11.Abstract:
Function of rotavirus NSP1 was analyzed by using single-NSP1 gene-substitution reassortants, SKF, SDF, and SNF which have the NSP1 gene derived from human rotaviruses KU, DS-1, and canine rotavirus K9, respectively, in the genetic background of simian rotavirus SAff11. The NSP1 genes from KU, DS-1, K9, and SAff11 exhibited 58–76% nucleotide sequence identity to one another. No substantial difference in viral growth was observed among the reassortants and SAff11. However, production of NSP1 was not detected in SNF when viral proteins were labelled with 35S-methionine during replication in MAff104 cells, in contrast to SAff11, SKF and SDF which exhibited evident expression of NSP1. Difference in reassortant formation was examined among the reassortant clones generated between human rotavirus strain 69M and either of SAff11, SKF or SNF. Although reassortant formation rate was significantly lower in the cross 69M × SNF than the other crosses, selection rates of RNA segments from parent strain 69M in the resultant reassortants was similar among the crosses. Selectivity of homolog- ous and heterologous NSP1 genes in SAff11 background was also analyzed by mixed infection and multiple passages among the single-NSP1 gene-reassortants and/or SAff11. KU NSP1 gene was selected most frequently, whereas homologous (SAff11) NSP1 gene was least efficiently segregated. These results indicated that viral growth and genome segment reassortment with other viruses may not be influenced by the presence of heterologous NSP1 and its expression level, while genomic diversity of NSP1 genes might have been associated with the relative adaptability to the genetic background of SAff11.read more
Citations
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Journal ArticleDOI
Effect of Intragenic Rearrangement and Changes in the 3′ Consensus Sequence on NSP1 Expression and Rotavirus Replication
John T. Patton,Zenobia F. Taraporewala,Dayue Chen,Vladimir Chizhikov,Melinda T. Jones,Azza Elhelu,Megan Collins,Karen Kearney,Mariam Wagner,Yasutaka Hoshino,Vera Gouvea +10 more
TL;DR: The results provide evidence that the 3′ consensus sequence of the gene 5 dsRNAs of SA11 rotaviruses has undergone a mutation causing it to operate suboptimally in RNA replication and in the expression of NSP1 during the virus life cycle.
Journal ArticleDOI
Rotavirus Antagonism of the Innate Immune Response
TL;DR: By antagonizing multiple components of the IFN-induction pathway, NSP1 aids viral spread and contributes to rotavirus pathogenesis.
Journal ArticleDOI
Cell-line-induced mutation of the rotavirus genome alters expression of an IRF3-interacting protein
Karen Kearney,Dayue Chen,Zenobia F. Taraporewala,Patrice Vende,Yasutaka Hoshino,Maria Alejandra Tortorici,Mario Barro,John T. Patton +7 more
TL;DR: It is found that the 3′CS of the gene (g5) encoding NSP1, an antagonist of interferon signaling, undergoes rapid mutation when rhesus rotavirus (RRV) is serially passaged at high multiplicity of infection (MOI) in cells permitting high titer growth.
Journal ArticleDOI
Analysis of genetic factors related to preferential selection of the NSP1 gene segment observed in mixed infection and multiple passage of rotaviruses.
TL;DR: Analysis of genetic factors which are associated with preferential selection of the rotavirus NSP1 gene segment into progeny viruses suggests that production of the intact NSP 1 protein may not be involved, but the presence of intact length of the N SP1 gene may be required.
Journal ArticleDOI
Rapid generation of rotavirus single-gene reassortants by means of eleven plasmid-only based reverse genetics
Saori Fukuda,Riona Hatazawa,Yoshiki Kawamura,Tetsushi Yoshikawa,Takayuki Murata,Koki Taniguchi,Satoshi Komoto +6 more
TL;DR: The results demonstrate that the 11 plasmid-only system allows the rapid and reliable generation of RVA single-gene reassortants, which will be useful for basic research and clinical applications.
References
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Journal ArticleDOI
Murine rotavirus genes encoding outer capsid proteins VP4 and VP7 are not major determinants of host range restriction and virulence.
TL;DR: It is concluded that the major outer capsid proteins VP4 and VP7 are not primarily responsible for virulence or host range restriction in the mouse model using a homologous murine rotavirus.
Journal ArticleDOI
Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection
Mario Gorziglia,Yasutaka Hoshino,Alicia Buckler-White,Ilse Blumentals,Roger I. Glass,Jorge Flores,Albert Z. Kapikian,Robert M. Chanock +7 more
TL;DR: The findings suggest that the fourth genes of virulent and asymptomatic human rotavirus strains represent two lines of divergent evolution from a common ancestor.
Journal ArticleDOI
Analysis of reassortment of genome segments in mice mixedly infected with rotaviruses SA11 and RRV.
J L Gombold,R F Ramig +1 more
TL;DR: A few reassortants with specific constellations of SA11 and RRV genome segments were repeatedly isolated from different litters or different animals within single litters, suggesting that these genotypes were independently and specifically selected in vivo.
Journal ArticleDOI
Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides, including a cysteine-rich zinc finger motif-encoding region (nucleotides 156 to 248), or which has a nonsense codon at nucleotides 153-155.
TL;DR: Two nondefective bovine rotavirus mutants (A5-10 and A5-16 clones) which have nonsense mutations in the early portion of the open reading frame of the NSP1 gene replicated well in cultured cells, although the plaque size of A 5-16 was extremely small.
Journal ArticleDOI
Comparison of the Rotavirus Nonstructural Protein NSP1 (NS53) from Different Species by Sequence Analysis and Northern Blot Hybridization
TL;DR: The species specificity of NSP1 is consistent with the hypothesis that N SP1 plays a role in host range restriction.
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