Journal ArticleDOI
Functional characterisation of a cytokinin oxidase gene DSCKX1 in Dendrobium orchid
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TLDR
DSCKX1 appears to play an important role on cytokinin metabolism and the related developmental programmes in orchid, as cloned by mRNA differential display from shoot apices of Dendrobium Sonia cultured in the presence of BA.Abstract:
Cytokinin oxidase plays an important role in the cytokinin regulatory processes We have cloned a novel putative cytokinin oxidase, DSCKX1 (Dendrobium Sonia cytokinin oxidase), by mRNA differential display from shoot apices of Dendrobium Sonia cultured in the presence of BA The DSCKX1 gene appears to have three alternative splicing forms and its expression of DSCKX1 was induced in a tissue-specific manner by cytokinins In transgenic orchid plants overexpressing DSCKX1, the elevated level of cytokinin oxidase activity was accompanied by a reduction of cytokinin content These plants exhibited slow shoot growth with numerous and long roots in vitro Their calli also showed decreased capability of shoot formation Conversly, antisense transgenic plants showed rapid proliferation of shoots and inhibition of root growth combined with a higher endogenous cytokinin content than wild-type plants Thus DSCKX1 appears to play an important role on cytokinin metabolism and the related developmental programmes in orchidread more
Citations
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Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity
TL;DR: The results are consistent with the hypothesis that cytokinins have central, but opposite, regulatory functions in root and shoot meristems and indicate that a fine-tuned control of catabolism plays an important role in ensuring the proper regulation of cytokinin functions.
Journal ArticleDOI
Arabidopsis Cytokinin Receptor Mutants Reveal Functions in Shoot Growth, Leaf Senescence, Seed Size, Germination, Root Development, and Cytokinin Metabolism
TL;DR: Researchers used loss-of-function mutants to study three Arabidopsis thaliana sensor histidine kinases to reveal partially redundant functions of the cytokinin receptors and prominent roles for the AHK2/AHK3 receptor combination in quantitative control of organ growth in plants, with opposite regulatory functions in roots and shoots.
Journal ArticleDOI
Structure and function of cytokinin oxidase/dehydrogenase genes of maize, rice, Arabidopsis and other species
TL;DR: The evolutionary development of cytokinin-catabolizing gene families and the individual properties of their members indicate an important role for the fine-tuned control of catabolism to assure proper regulation of cytkinin functions.
Journal ArticleDOI
New Insights into the Biology of Cytokinin Degradation
TL;DR: The results corroborate the relevance of metabolic control in determining cytokinin activity and show using reporter gene and semiquantitative RT-PCR analyses regulation of individual CKX genes by cytokinIn, auxin, ABA, and phosphate starvation.
Journal ArticleDOI
TaCKX6‐D1, the ortholog of rice OsCKX2, is associated with grain weight in hexaploid wheat
TL;DR: In this paper, a wheat ortholog of rice OsCKX2 was cloned and its haplotype variants were determined to be significantly associated with the 1000-grain weight on the basis of linkage mapping, association analysis and gene expression analysis.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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TL;DR: A method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction using a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer.
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Regulation of plant growth by cytokinin
TL;DR: It is suggested that cytokinins are an important regulatory factor of plant meristem activity and morphogenesis, with opposing roles in shoots and roots.
Journal ArticleDOI
The Biosynthesis and Metabolism of Cytokinins
and D S Letham,Lok Man S. Palni +1 more