Functions of coated vesicles during protein absorption in the rat vas deferens
Reads0
Chats0
TLDR
Large coated vesicles serve as heterophagosomes to transport absorbed protein to lysosomes, and some small coated vedicle serve as primary lysOSome to transport hydrolytic enzymes from the Golgi complex to multivesicular bodies.Abstract:
The role of coated vesicles during the absorption of horseradish peroxidase was investigated in the epithelium of the rat vas deferens by electron microscopy and cytochemistry. Peroxidase was introduced into the vas lumen in vivo. Tissue was excised at selected intervals, fixed in formaldehyde-glutaraldehyde, sectioned without freezing, incubated in Karnovsky's medium, postfixed in OsO(4), and processed for electron microscopy. Some controls and peroxidase-perfused specimens were incubated with TPP,(1) GP, and CMP. Attention was focused on the Golgi complex, apical multivesicular bodies, and two populations of coated vesicles; large (> 1000 A) ones concentrated in the apical cytoplasm and small (<750 A) ones found primarily in the Golgi region. 10 min after peroxidase injection, the tracer is found adhering to the surface plasmalemma, concentrated in bristle-coated invaginations, and within large coated vesicles. After 20-45 min, it is present in large smooth vesicles, apical multivesicular bodies, and dense bodies. Peroxidase is not seen in small coated vesicles at any interval. Counts of small coated vesicles reveal that during peroxidase absorption they first increase in number in the Golgi region and later, in the apical cytoplasm. In both control and peroxidase-perfused specimens incubated with TPP, reaction product is seen in several Golgi cisternae and in small coated vesicles in the Golgi region. With GP, reaction product is seen in one to two Golgi cisternae, multivesicular bodies, dense bodies, and small coated vesicles present in the Golgi region or near multivesicular bodies. The results demonstrate that (a) this epithelium functions in the absorption of protein from the duct lumen, (b) large coated vesicles serve as heterophagosomes to transport absorbed protein to lysosomes, and (c) some small coated vesicles serve as primary lysosomes to transport hydrolytic enzymes from the Golgi complex to multivesicular bodies.read more
Citations
More filters
Journal ArticleDOI
[Golgi apparatus in ciliates. Ultrastructure, with special reference to Paramecium].
TL;DR: Electronmicroscopic study of Coleps, Colpidium, Stylonychia, and especially of Paramecium confirmed the presence of the Golgi complex in these fresh-water ciliates, which consisted of numerous dictyosomes scattered throughout the cytoplasm.
Journal ArticleDOI
Uptake and intracellular distribution of ferritin in the rat distal convoluted tubule
TL;DR: It is revealed that in the DCT, CF is bound to the apical cell membrane and taken up into the tubule cells, whereas only traces of AF are taken up, indicating that the charge of a protein molecule may determine whether or not the protein is reabsorbed by the D CT.
Journal ArticleDOI
Involvement of the Golgi apparatus in sorting of materials to opposite ends of frog rod retinal photoreceptors.
Robert Schmied,Eric Holtzman +1 more
TL;DR: The Golgi apparatus of these cells has a cis-trans organization like that of other neurons as regards morphological features and the distribution of phosphatase activities, and the findings suggest that sorting of membrane proteins destined for opposite ends of the photoreceptor takes place in systems at or near the trans Golgi face.
Journal ArticleDOI
Die endocytose-phase der eiwei\resorption im proximalen nierentubulus
W. Thoenes,K. H. Langer +1 more
TL;DR: In this article, Ferritin is used as Testeiweis in das Lumen proximaler Tubuluskonvolutschlingen der Rattenniere injiziert Nach unterschiedlichen Resorptionszeiten werden die Tubuli intravital fixiert and elektronenmikroskopisch untersucht.
Journal ArticleDOI
Light-induced changes in the ultrastructure of a plasmodial myxomycete.
J.W. Daniel,Ulla Järlfors +1 more
TL;DR: Light required to induce sexual sporulation (fruiting) of the plasmodial myxomycete Physaruin polycephalum causes the formation of electron-dense deposits in the intermembrane spaces of mitochondria and in cytoplasmic vacuoles, rich in calcium.
References
More filters
Journal ArticleDOI
Improvements in epoxy resin embedding methods.
TL;DR: Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenientembedding methods for electron microscopy.
Journal Article
A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron-microscopy
Journal ArticleDOI
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
TL;DR: The early stages of absorption of intravenously injected horseradish peroxidase in proximal tubules of mouse kidney were studied with a new ultrastructural cytochemical technique, which gives sharp localization and is sensitive to protein transport.
Journal ArticleDOI
Junctional complexes in various epithelia
TL;DR: The tight junction is impervious to concentrated protein solutions and appears to function as a diffusion barrier or "seal," and the desmosome and probably also the zonula adhaerens may represent intercellular attachment devices.
Related Papers (5)
Yolk protein uptake in the oocyte of the mosquito aedes aegypti. l.
Thomas F. Roth,Keith R. Porter +1 more