Functions of coated vesicles during protein absorption in the rat vas deferens
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Large coated vesicles serve as heterophagosomes to transport absorbed protein to lysosomes, and some small coated vedicle serve as primary lysOSome to transport hydrolytic enzymes from the Golgi complex to multivesicular bodies.Abstract:
The role of coated vesicles during the absorption of horseradish peroxidase was investigated in the epithelium of the rat vas deferens by electron microscopy and cytochemistry. Peroxidase was introduced into the vas lumen in vivo. Tissue was excised at selected intervals, fixed in formaldehyde-glutaraldehyde, sectioned without freezing, incubated in Karnovsky's medium, postfixed in OsO(4), and processed for electron microscopy. Some controls and peroxidase-perfused specimens were incubated with TPP,(1) GP, and CMP. Attention was focused on the Golgi complex, apical multivesicular bodies, and two populations of coated vesicles; large (> 1000 A) ones concentrated in the apical cytoplasm and small (<750 A) ones found primarily in the Golgi region. 10 min after peroxidase injection, the tracer is found adhering to the surface plasmalemma, concentrated in bristle-coated invaginations, and within large coated vesicles. After 20-45 min, it is present in large smooth vesicles, apical multivesicular bodies, and dense bodies. Peroxidase is not seen in small coated vesicles at any interval. Counts of small coated vesicles reveal that during peroxidase absorption they first increase in number in the Golgi region and later, in the apical cytoplasm. In both control and peroxidase-perfused specimens incubated with TPP, reaction product is seen in several Golgi cisternae and in small coated vesicles in the Golgi region. With GP, reaction product is seen in one to two Golgi cisternae, multivesicular bodies, dense bodies, and small coated vesicles present in the Golgi region or near multivesicular bodies. The results demonstrate that (a) this epithelium functions in the absorption of protein from the duct lumen, (b) large coated vesicles serve as heterophagosomes to transport absorbed protein to lysosomes, and (c) some small coated vesicles serve as primary lysosomes to transport hydrolytic enzymes from the Golgi complex to multivesicular bodies.read more
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Book ChapterDOI
[1] Intracellular membrane traffic: Pathways, carriers, and sorting devices
TL;DR: It has become clear that cells repeatedly reutilize or recycle the vesicular membranes involved in carrying out these various transport operations, and the Golgi complex is the intracellular site where much of the biosynthetic and recycling membrane traffic converges and where products are sorted and directed to their correct destinations.
Book
Functional Morphology of the Golgi Apparatus
TL;DR: This chapter discusses Golgi Architecture in the Course of Cell Differentiation, Morphology and Classic Cytochemistry, and Variability of the Golgi architecture.
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The mucigenous glandular mucosa in the complex stomach of two new-world camelids, the llama and guanaco†
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References
More filters
Journal ArticleDOI
Improvements in epoxy resin embedding methods.
TL;DR: Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenientembedding methods for electron microscopy.
Journal Article
A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron-microscopy
Journal ArticleDOI
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
TL;DR: The early stages of absorption of intravenously injected horseradish peroxidase in proximal tubules of mouse kidney were studied with a new ultrastructural cytochemical technique, which gives sharp localization and is sensitive to protein transport.
Journal ArticleDOI
Junctional complexes in various epithelia
TL;DR: The tight junction is impervious to concentrated protein solutions and appears to function as a diffusion barrier or "seal," and the desmosome and probably also the zonula adhaerens may represent intercellular attachment devices.
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