Journal ArticleDOI
Genetic transformation of Brassica campestris var. rapa protoplasts with an engineered cauliflower mosaic virus genome.
TLDR
A hybrid CaMV genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3′)II] gene from Tn5.Abstract:
A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3′)II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplast transformation system of Brassica campestris var. rapa. Stable, genetically transformed cell lines of B. campestris var. rapa were obtained after transformation. DNA of the hybrid CaMV genome was found to be integrated into high molecular weight plant genomic DNA. Transformation was achieved only when the hybrid genome was supplied together with wild type viral DNA. A possible complementation of the modified CaMV genome with the wild type viral DNA as a helper molecule in planta and in the protoplast system is discussed.read more
Citations
More filters
Journal Article
Chimeric genes as dominant selectable markers in plant cells
TL;DR: A chimeric gene was constructed consisting of the promoter of the nopaline synthase gene of Agrobacterium and the structural gene of the aminoglycoside phosphotransferase of Tn5.
Patent
Transformation and foreign gene expression in brassica species
Maurice M. Moloney,Sharon Radke +1 more
TL;DR: In this paper, the Brassica species are transformed employing a manipulated Agrobacterium transformation system, followed by regeneration of the plant tissue into plants, and the cells and the plants produced thereby are capable of expressing the foreign gene.
Journal ArticleDOI
Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts
Hongmei Zhang,H. Yang,Elíbio L. Rech,T. J. Golds,A. S. Davis,Bernard J. Mulligan,Edward C. Cocking,Michael R. Davey +7 more
TL;DR: This report of the production of kanamycin resistant transgenic rice plants establishes the use of protoplasts for rice genetic engineering.
Journal ArticleDOI
Efficient Transformation of Arabidopsis-Thaliana Using Direct Gene-Transfer to Protoplasts
TL;DR: Transformation experiments performed with a selectable and a non-selectable gene on separate plasmids resulted in a co-transformation rate of functionally active copies in about 25% of the transformants analysed, suggesting this approach can be used to introduce non- selectable genes into the Arabidopsis genome.
Journal ArticleDOI
Transgenic Plants of Brassica napus L.
TL;DR: Longitudinal stem sections of Brassica napus L. cv.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
TL;DR: The effects of 5′ deletions in a plant viral promoter in tobacco callus as well as in regenerated plants, includ ing different plant tissues, are analysed to allow a more direct assessment of deletion effects.
Journal ArticleDOI
Expression of bacterial genes in plant cells.
Robert T. Fraley,Stephen G. Rogers,Robert B. Horsch,Patricia R. Sanders,Jeffery S. Flick,Steven Paul Adams,Michael L. Bittner,Leslie Brand,Cynthia L. Fink,Joyce S. Fry,Gerald R. Galluppi,Sarah B. Goldberg,Nancy L. Hoffmann,Sherry C. Woo +13 more
TL;DR: Chimeric bacterial genes conferring resistance to aminoglycoside antibiotics have been inserted into the Agrobacterium tumefaciens tumor-inducing (Ti) plasmid and introduced into plant cells by in vitro transformation techniques.
Journal ArticleDOI
Direct gene transfer to plants.
Jerzy Paszkowski,Raymond D. Shillito,Michael W. Saul,Vaclav Mandak,Thomas Hohn,Barbara Hohn,Ingo Potrykus +6 more
TL;DR: Plants regenerated from transformed cell lines were phenotypically normal and fertile, and they maintained and expressed the foreign gene throughout the development of vegetative and generative organs.