Genomic epidemiology and characterisation of penicillin-sensitive Staphylococcus aureus isolates from invasive bloodstream infections in China: an increasing prevalence and higher diversity in genetic typing be revealed
TLDR
Virulence assays showed that MSSA-PENS exhibited a level of virulence comparable to that of penicillin-resistant MSSA (MSSA- PENR), indicating that more-sensitive strains should not be mistaken for lacking aggressiveness in vivo.Abstract:
ABSTRACT Many countries have reported increasing rates of penicillin-susceptible methicillin-sensitive Staphylococcus aureus (MSSA-PENS). To date, there is relatively little known about the current situation and molecular characteristics of MSSA-PENS in China. In this study, we carried out a laboratory-based multi-region retrospective study to investigate the genomic epidemiology and characterisation of MSSA-PENS isolated from invasive bloodstream infections (BSIs) across 17 provinces. The prevalence of MSSA-PENS isolates increased significantly over the 6-year period, with the proportion increasing from 3.51% in 2014–8.80% in 2019, an average relative increase of 22.14% per year (95% confidence interval 9.67%-34.61%, P for trend <0.001), suggesting that China is experiencing a resurgence of MSSA-PENS. Phylogenetic analysis showed a higher strain diversity occurred; the most frequent clonal complexes (CCs) identified were CC188 (17.14%), CC398 (15.71%) and CC5 (15.71%). Over half of MSSA-PENS strains were pan-susceptible, with erythromycin the most frequent resistance observed. Moreover, 25 isolates were identified as immune evasion cluster negative, including CC15, CC188 and CC1, and 6 strains encoded the Panton-Valentine leucocidin gene. Importantly, virulence assays showed that MSSA-PENS exhibited a level of virulence comparable to that of penicillin-resistant MSSA (MSSA-PENR), indicating that more-sensitive strains should not be mistaken for lacking aggressiveness in vivo. Furthermore, 11 of these isolates were confirmed as blaZ positive but phenotype sensitive, with different amino acid changes in blaZ. Our data support the recommendation to clinicians regarding the usage of penicillin in invasive BSIs caused by MSSA-PENS, which might create a novel opportunity for better antimicrobial stewardship in the future.read more
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References
More filters
Journal ArticleDOI
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.
TL;DR: This work presents some of the most notable new features and extensions of RAxML, such as a substantial extension of substitution models and supported data types, the introduction of SSE3, AVX and AVX2 vector intrinsics, techniques for reducing the memory requirements of the code and a plethora of operations for conducting post-analyses on sets of trees.
Journal ArticleDOI
fastp: an ultra-fast all-in-one FASTQ preprocessor.
TL;DR: Fastp is developed as an ultra‐fast FASTQ preprocessor with useful quality control and data‐filtering features that can perform quality control, adapter trimming, quality filtering, per‐read quality pruning and many other operations with a single scan of the FAST Q data.
Journal ArticleDOI
Staphylococcus aureus infections.
TL;DR: In an elegant series of clinical observations and laboratory studies published in 1880 and 1882, Ogston described staphylococcal disease and its role in sepsis and abscess formation.
Posted ContentDOI
fastp: an ultra-fast all-in-one FASTQ preprocessor
TL;DR: Fastp is developed as an ultra-fast FASTQ preprocessor with useful quality control and data-filtering features that can perform quality control, adapter trimming, quality filtering, per-read quality cutting, and many other operations with a single scan of the FastQ data.
Journal ArticleDOI
Roary: Rapid large-scale prokaryote pan genome analysis
Andrew J. Page,Carla A. Cummins,Martin Hunt,Vanessa K. Wong,Sandra Reuter,Matthew T. G. Holden,Maria Fookes,Daniel Falush,Jacqueline A. Keane,Julian Parkhill +9 more
TL;DR: Roary, a tool that rapidly builds large-scale pan genomes, identifying the core and accessory genes, is introduced, making construction of the pan genome of thousands of prokaryote samples possible on a standard desktop without compromising on the accuracy of results.