Gibberellin 20-Oxidase Gene OsGA20ox3 Regulates Plant Stature and Disease Development in Rice
Reads0
Chats0
TLDR
The results provide direct evidence for a critical role of OsGA20ox3 in regulating not only plant stature but also disease resistance in rice.Abstract:
Gibberellin (GA) 20-oxidase (GA20ox) catalyses consecutive steps of oxidation in the late part of the GA biosynthetic pathway. A T-DNA insertion mutant (17S-14) in rice, with an elongated phenotype, was isolated. Analysis of the flanking sequences of the T-DNA insertion site revealed that an incomplete T-DNA integration resulted in enhanced constitutively expression of downstream OsGA20ox3 in the mutant. The accumulation of bioactive GA(1) and GA(4) were increased in the mutant in comparison with the wild-type plant. Transgenic plants overexpressing OsGA20ox3 showed phenotypes similar to those of the 17S-14 mutant, and the RNA interference (RNAi) lines that had decreased OsGA20ox3 expression exhibited a semidwarf phenotype. Expression of OsGA20ox3 was detected in the leaves and roots of young seedlings, immature panicles, anthers, and pollens, based on β-glucuronidase (GUS) activity staining in transgenic plants expressing the OsGA20ox3 promoter fused to the GUS gene. The OsGA20ox3 RNAi lines showed enhanced resistance against rice pathogens Magnaporthe oryzae (causing rice blast) and Xanthomonas oryzae pv. oryzae (causing bacterial blight) and increased expression of defense-related genes. Conversely, OsGA20ox3-overexpressing plants were more susceptible to these pathogens comparing with the wild-type plants. The susceptibility of wild-type plants to X. oryzae pv. oryzae was increased by exogenous application of GA(3) and decreased by S-3307 treatment. Together, the results provide direct evidence for a critical role of OsGA20ox3 in regulating not only plant stature but also disease resistance in rice.read more
Citations
More filters
Journal ArticleDOI
Intervention of Phytohormone Pathways by Pathogen Effectors
Kemal Kazan,Rebecca Lyons +1 more
TL;DR: An improved understanding of phytohormone intervention strategies employed by pests and pathogens during their interactions with plants will ultimately lead to the development of new crop protection strategies.
Journal ArticleDOI
Roles of plant hormones and their interplay in rice immunity.
TL;DR: The roles of plant hormones in rice immunity are summarized, their interplay/crosstalk mechanisms and the complex regulatory network of plant hormone pathways in fine-tuning rice immunity and growth are discussed.
Journal ArticleDOI
Connecting Growth and Defense: The Emerging Roles of Brassinosteroids and Gibberellins in Plant Innate Immunity
TL;DR: Interactions between BR and GA signaling, plant defense, and microbial virulence are highlighted and a crucial role of GA-inhibiting DELLA proteins and the BR-regulated transcription factor BZR1 are identified.
Journal ArticleDOI
Hormone defense networking in rice: tales from a different world
TL;DR: The roles of both classical and more recently identified stress hormones in regulating immunity in the model monocot rice (Oryza sativa) are reviewed and the importance of hormone crosstalk in shaping the outcome of rice-pathogen interactions is highlighted.
Journal ArticleDOI
Making sense of hormone-mediated defense networking: from rice to Arabidopsis.
TL;DR: This review addresses the latest discoveries dealing with hormone-mediated immunity in rice, one of the most important food crops and an excellent model for molecular genetic studies in monocots, and highlights interactions between hormone signaling, rice defense and pathogen virulence.
References
More filters
Journal ArticleDOI
Gibberellin Metabolism and its Regulation
TL;DR: Current understanding of the GA biosynthesis and deactivation pathways in plants and fungi is summarized, and how GA concentrations in plant tissues are regulated during development and in response to environmental stimuli is discussed.
Journal ArticleDOI
Efficient isolation and mapping of Arabidopsis thaliana T‐DNA insert junctions by thermal asymmetric interlaced PCR
TL;DR: Thermal asymmetric interlaced PCR is an efficient technique for amplifying insert ends from yeast artificial chromosome and P1 clones and the adaptation of this method for recovery and mapping of genomic sequences flanking T-DNA insertions in Arabidopsis thaliana is described.
Journal ArticleDOI
GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin
Miyako Ueguchi-Tanaka,Motoyuki Ashikari,Masatoshi Nakajima,Hironori Itoh,Etsuko Katoh,Masatomo Kobayashi,Teh-yuan Chow,Teh-yuan Chow,Yue-ie C Hsing,Hidemi Kitano,Isomaro Yamaguchi,Makoto Matsuoka +11 more
TL;DR: The results indicate that GID1 is a soluble receptor mediating GA signalling in rice, a new GA-insensitive dwarf mutant of rice, that encodes an unknown protein with similarity to the hormone-sensitive lipases.
Journal ArticleDOI
Gibberellin signaling: biosynthesis, catabolism, and response pathways.
TL;DR: The power of molecular genetics has dramatically advanced the understanding of all aspects of gibberellin signaling, and many genes encoding GA response pathway components have been identified using Arabidopsis and cereal mutants.
Journal ArticleDOI
Gibberellin metabolism: new insights revealed by the genes.
Peter Hedden,Andrew L. Phillips +1 more
TL;DR: Several endogenous and environmental factors modify the expression of gibberellin biosynthesis genes, including developmental stage, hormonal status and light, adding unexpected genetic complexity.