Journal ArticleDOI
Glycosis in the eggs of the echiuroid, Urechis unicinctus and the oyster, Crassostrea gigas. Rate-limiting steps and activation at fertilization.
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TLDR
Calculation of the mass action ratio from the amounts of glycolytic intermediates determined suggests that there are at least three limiting enzymes in the gly colytic system in unfertilized and fertilized eggs of each species examined, and phosphorylase, phosphofructokinase, and pyruvate kinase may be rate-limiting enzymes for the Glycolysis system in Urechis eggs as well as in oyster eggs.About:
This article is published in Experimental Cell Research.The article was published on 1975-06-01. It has received 13 citations till now. The article focuses on the topics: Urechis unicinctus & Phosphofructokinase activity.read more
Citations
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Journal ArticleDOI
Regulation of glucose metabolism by adenine nucleotides in round spermatids from rat testes.
TL;DR: Glyceraldehyde-3-phosphate dehydrogenase is inhibited by the adenine nucleotides, particularly by 5'-AMP and ADP as inhibitors competitive with NAD.
Journal ArticleDOI
Metabolism of round spermatids: evidence that lactate is preferred substrate.
TL;DR: Observations further support the hypothesis that lactate is the preferred substrate for spermatid metabolism, and the importance of LDH in glucose and lactate metabolism by sper matids was described.
Journal ArticleDOI
Integrating Discovery-driven Proteomics and Selected Reaction Monitoring To Develop a Noninvasive Assay for Geoduck Reproductive Maturation
Emma Timmins-Schiffman,Grace A. Crandall,Brent Vadopalas,Michael Riffle,Brook L. Nunn,Steven B. Roberts +5 more
TL;DR: Application of SRM in hemolymph samples demonstrates that this procedure could effectively be used to assess reproductive status in marine mollusks in a nonlethal manner.
Journal ArticleDOI
Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis.
TL;DR: In the DGE database of U. unicinctus, the alterations in certain known sulfide-related pathways indicate similar changes in response to sulfide, and this is the first report on their association with sulfide stress.
Journal ArticleDOI
Enzyme activities in the glycolysis system of Urechis eggs with special reference to the activation of its rate-limiting enzymes following fertilization.
TL;DR: In fertilized eggs of Urechis unicinctus, the activities of phosphorylase, phosphofructokinase, and pyruvate kinase are markedly higher than those in unfertilized eggs, which will be ascribed to the increase in cAMP concentration following fertilization.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The Redox State of Free Nicotinamide-Adenine Dinucleotide in the Cytoplasm and Mitochondria of Rat Liver
TL;DR: The bearing of these findings on various problems, including the number of NAD(+)-NADH pools in liver cells; the applicability of the method to tissues other than liver; the transhydrogenase activity of glutamate dehydrogenase; the physiological significance of the difference of the redox states of mitochondria and cytoplasm; aspects of the regulation of theredox state of cell compartments; the steady-state concentration of mitochondrial oxaloacetate.
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Purification and allosteric properties of yeast pyruvate kinase.
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Periodic change in the content of adenosine 3′5′-cyclic monophosphate with close relation to the cycle of cleavage in the sea urchin egg
TL;DR: The periodic change in cAMP content in the sea urchin egg seems to be repeated with close relation to the cycle of cytokinesis.
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