Glyoxalase III from Escherichia coli: a single novel enzyme for the conversion of methylglyoxal into d-lactate without reduced glutathione
TLDR
The p-hydroxymercuribenzoate-inactivated enzyme could be almost completely re-activated by dithiothreitol and other thiol-group-containing compounds, indicating the possible involvement of thiol group(s) at or near the active site of the enzyme.Abstract:
A single novel enzyme, glyoxalase III, which catalyses the conversion of methylglyoxal into D-lactate without involvement of GSH, has been detected in and purified from Escherichia coli Of several carbonyl compounds tested, only the alpha-ketoaldehydes methylglyoxal and phenylglyoxal were found to be substrates for this enzyme Glyoxalase III is active over a wide range of pH with no sharp pH optimum In its native form it has an M(r) of 82000 +/- 2000, and it is composed of two subunits of equal M(r) Glutathione analogues, which are inhibitors of glyoxalase I, do not inhibit glyoxalase III Glyoxalase III is found to be sensitive to thiol-blocking reagents The p-hydroxymercuribenzoate-inactivated enzyme could be almost completely re-activated by dithiothreitol and other thiol-group-containing compounds, indicating the possible involvement of thiol group(s) at or near the active site of the enzymeread more
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Journal ArticleDOI
Methylglyoxal in living organisms: chemistry, biochemistry, toxicology and biological implications.
TL;DR: A comprehensive overview of methylglyoxal research, extending discussion from chemistry to biological implications by reviewing some important characteristics of methyl glyoxal metabolism and toxicity in a wide variety of species, and emphasizing the action of methyl-oxoaldehyde production in the environment as a potential risk factor and to the possible role of this a-dicarbonyl in diseases as discussed by the authors.
Journal ArticleDOI
Methylglyoxal, a highly reactive dicarbonyl compound, in diabetes, its vascular complications and other age-related diseases
TL;DR: The mechanisms through which MGO is formed, its detoxification by the glyoxalase system, and its effect on biochemical pathways in relation to the development of diabetes, vascular complications of diabetes and other age-related diseases are summarized.
Journal ArticleDOI
Methylglyoxal production in bacteria: suicide or survival?
TL;DR: Analysis of a methylglyoxal synthase-deficient mutant provides evidence that methylgly oxal production is required to allow growth under certain environmental conditions, and may represent a high-risk strategy that facilitates adaptation, but which on failure leads to cell death.
Journal ArticleDOI
The role of methylglyoxal and the glyoxalase system in diabetes and other age-related diseases.
TL;DR: The present review summarizes the mechanisms through which MGO is formed, its detoxification by the glyoxalase system and its effect on biochemical pathways in relation to the development of age-related diseases.
Journal ArticleDOI
Human DJ-1 and its homologs are novel glyoxalases
Juyoung Lee,Jeeyeon Song,Kyu Kwon,Sumi Jang,Chayeon Kim,Kwanghee Baek,Jeongho Kim,Chankyu Park +7 more
TL;DR: It is reported here that human DJ-1 and its homologs of the mouse and Caenorhabditis elegans are novel types of glyoxalase, converting Glyoxal or methylglyoxal to glycolic or lactic acid, respectively, in the absence of glutathione.
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Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.