Proceedings ArticleDOI
GPC-modalities for Neurophotonics and Optogenetics
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TLDR
This work will report on the latest laser shaping GPC-modalities with temporal focusing to uniquely sculpt patterns of one- and two-photon excitations for living neural-circuits and genetically modified light-sensitive cells.Abstract:
The powerful discipline of optogenetics can be described as “the branch of biotechnology which combines genetic engineering with optics to observe and control the function of genetically targeted groups of cells with light”. Using advanced laser beam-shaping such as Generalized Phase Contrast (GPC) and derived holographic modalities makes it possible to take advantage of cutting-edge two-photon technology to develop an unprecedented ‘circuit optogenetics’ platform with both high spatio-temporal selectivity and high penetration depth without disturbing speckle-noise. We will report on the latest laser shaping GPC-modalities with temporal focusing to uniquely sculpt patterns of one- and two-photon excitations for living neural-circuits and genetically modified light-sensitive cells.read more
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Journal ArticleDOI
Differentiable Microscopy Designs an All Optical Quantitative Phase Microscope
Kithmini Herath,Udith Haputhanthri,Ramith Hettiarachchi,Hasindu Kariyawasam,Azeem Ahmad,Balpreet Singh Ahluwalia,Chamira U. S. Edussooriya,Dushan N. Wadduwage +7 more
TL;DR: In this article , the authors propose a solution to solve the problem of the problem: REINFORCE/RESUME 7, 2019. . . . , . . ) .
References
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Journal ArticleDOI
Scanless two-photon excitation of channelrhodopsin-2
Eirini Papagiakoumou,Francesca Anselmi,Aurélien Bègue,Vincent de Sars,Jesper Glückstad,Ehud Y. Isacoff,Ehud Y. Isacoff,Valentina Emiliani +7 more
TL;DR: A method that combines generalized phase contrast with temporal focusing (TF-GPC) to shape two-photon excitation for this purpose and generated large photocurrents in Channelrhodopsin-2–expressing cultured cells and neurons and in mouse acute cortical slices is developed.
Book
Generalized Phase Contrast:: Applications in Optics and Photonics
Jesper Glückstad,Darwin Palima +1 more
TL;DR: Wavefront Sensing and Analysis Using GPC, GPC-Based Programmable Optical Micromanipulation, and alternative GPC Schemes are studied.
Journal ArticleDOI
GPC light shaper: static and dynamic experimental demonstrations
TL;DR: This work experimentally verify previously derived conditions for photon-efficient light shaping with GPC, and demonstrates a compact implementation of GPC for creating practical illumination shapes that can find use in light-efficient industrial or commercial applications.
Journal ArticleDOI
Holo-GPC: Holographic Generalized Phase Contrast
TL;DR: In this paper, a hybrid of holography and generalized phase contrast (GPC) is proposed, which can efficiently control both the individual shape and the collective distribution of the resulting light beams.
Journal ArticleDOI
Matched-filtering generalized phase contrast using LCoS pico-projectors for beam-forming
TL;DR: A new beam-forming system for generating high intensity programmable optical spikes using so-called matched-filtering Generalized Phase Contrast (mGPC) applying two consumer handheld pico-projectors, presenting a low-cost alternative for optical trapping and manipulation, optical lattices and other beam-shaping applications usually implemented with high-end spatial light modulators.