Journal ArticleDOI
Growth and chemotherapeutic response in athymic mice of tumors arising from human glioma-derived cell lines.
TLDR
This model allows cell lines derived from human gliomas to be grown in animal hosts, thereby providing a potential means for evaluating growth parameters and chemotherapeutic responsiveness of tumors derived from individual humangliomas or cell lines.Abstract:
Fifteen permanent cell lines derived from human gliomas were subcutaneously transplanted into athymic nude mice (nu/nu genotype, NIH Swiss and BALB/c backgrounds). Four were tumorigenic. Three of the four (D-54 MG, U-118 MG, and U-251 MG) produced progressively growing, solid, noncystic tumors. Subcutaneous volume measurement of these tumors, which correlated directly with tumor weight, was a reliable method for monitoring growth. All three cell lines which produced progressively growing subcutaneous tumors were also tumorigenic when cells were inoculated intracerebrally. These grew as well-circumscribed, intraparenchymal brain tumors. After initial implantation, each of the progressively growing, solid, subcutaneous tumors was histologically similar to the permanent cell lines from which it was derived. Tumors could be reliably passed, and stabilization of latency periods and growth rates developed. Tumors became morphologically less distinct in later passages, though some individual features remained. Mice bearing subcutaneous tumors from each of these cell lines were treated with a single ip dose of 25 mg/kg BCNU and compared to controls receiving only drug vehicle. A significant, but different, amount of reduction in tumor mass occurred among each of the three tumor lines. This model allows cell lines derived from human gliomas to be grown in animal hosts, thereby providing a potential means for evaluating growth parameters and chemotherapeutic responsiveness of tumors derived from individual human gliomas or cell lines.read more
Citations
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Journal ArticleDOI
Determination of subcutaneous tumor size in athymic (nude) mice
TL;DR: In this article, the authors compared the use of 19 different formulas for determining the size of subcutaneous tumors grown as xenografts in nude mice (2 for determining tumor area, 3 for tumor diameter, and 14 for calculating tumor volume).
Journal ArticleDOI
Tumor measurement in the nude mouse.
TL;DR: In this study, the pitfalls of tumor measurement in the nude mouse were evaluated and recommendations are made for future work employing tumor measurement.
Journal ArticleDOI
Cerebral vasospasm after subarachnoid hemorrhage: putative role of inflammation.
Aaron S. Dumont,Randall J. Dumont,Michael M. Chow,Chi-lung Lin,Tarkan Calisaneller,Klaus Ley,Neal F. Kassell,Kevin S. Lee +7 more
TL;DR: A burgeoning body of evidence suggests that various constituents of the inflammatory response, including adhesion molecules, cytokines, leukocytes, immunoglobulins, and complement, may be critical in the pathogenesis of cerebral vasospasm as mentioned in this paper.
Journal ArticleDOI
Computerized tomographic and pathologic studies of the untreated, quiescent, and recurrent glioblastoma multiforme
Peter C. Burger,P. J. Dubois,S. C. Schold,K. R. Smith,Guy L. Odom,D. C. Crafts,Felice Giangaspero +6 more
TL;DR: Pathological findings in 20 cases of glioblastoma multiforme were correlated with clinical histories and computerized tomographic (CT) scans to define the neoplasm in three stages: before treatment, during remission, and during recurrence.
Patent
Controlled local delivery of chemotherapeutic agents for treating solid tumors
TL;DR: In this paper, a method and devices for localized delivery of a chemotherapeutic agent to solid tumors, wherein the agent does not cross the blood-brain barrier and is characterized by poor bioavailability and/or short half-lives in vivo, are described.