Herpes simplex virus 1 protein kinase is encoded by open reading frame US3 which is not essential for virus growth in cell culture.
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The results lead to the conclusion that the US3 open reading frame encodes a virus-specific protein kinase that is not required for virus growth in cells in culture.Abstract:
Earlier reports have described a novel protein kinase in cells infected with herpes simplex or pseudorabies viruses. These novel enzymes were characterized by their acceptance of protamine as a substrate and by their differential chromatographic behavior in anion-exchange chromatography. We report that this activity was not present in extracts of uninfected cells or of cells infected with a mutant constructed so as to contain a deletion in the US3 open reading frame mapping in the small component of herpes simplex virus 1 DNA. The activity was present in extracts of cells infected with wild-type virus and with a recombinant in which the US3 open reading frame had been rescued. Our results are consistent with the observation reported earlier that the coding sequences predict an amino acid motif common to protein kinases and lead to the conclusion that the US3 open reading frame encodes a virus-specific protein kinase that is not required for virus growth in cells in culture.read more
Citations
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Journal ArticleDOI
Herpesvirus Assembly and Egress
TL;DR: Findings are focused on recent findings that demonstrate a rather complex process of herpesvirus maturation including primary envelopment of capsids by budding at the inner leaflet of the nuclear membrane and translocation of capsid into the cytoplasm after loss of the primary envelope by fusion with the outer leaflets of thenuclear membrane.
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Ultrastructural localization of the herpes simplex virus type 1 UL31, UL34, and US3 proteins suggests specific roles in primary envelopment and egress of nucleocapsids.
TL;DR: The data support the herpes simplex virus type 1 deenvelopment-reenvelopment model of virion egress and suggest that the US3 protein plays an important, but nonessential, role in the egress pathway.
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U(L)31 and U(L)34 proteins of herpes simplex virus type 1 form a complex that accumulates at the nuclear rim and is required for envelopment of nucleocapsids.
TL;DR: Data strongly suggest that the UL31 and UL34 proteins form a complex that accumulates at the nuclear membrane and plays an important role in nucleocapsid envelopment at the inner nuclear membrane.
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Budding events in herpesvirus morphogenesis.
TL;DR: This review summarizes recent advances in the understanding of the two herpesvirus budding events, which are distinct not only in the subcellular compartments in which they occur but also by the viral proteins involved.
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The herpes simplex virus 1 protein kinase US3 is required for protection from apoptosis induced by the virus.
TL;DR: Results point to the protein kinase encoded by the US3 gene as the principal viral product required to block apoptosis.
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Characterization of Herpes Simplex Virus Strains Differing in their Effects on Social Behaviour of Infected Cells
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