Journal ArticleDOI
High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena
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TLDR
The results indicated that almost no somaclonal variation was detected among the regenerants and the genetic stability of the regenerated plants was analyzed by flow cytometry, RAPD and SSR molecular markers.Abstract:
A novel protocol for plant regeneration from cotyledon explants of eggplant (Solanum melongena) reducing concentration of sucrose was established. The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium supplemented with 2.0 mg dm−3 zeatin, 0.1 mg dm−3 indoleacetic acid and 10 g dm−3 sucrose. After 15 d, the shoot buds were fragmented and transferred to the shoot elongation MS supplemented with 1.0–2.0 mg dm−3 gibberellic acid and 4.0–8.0 mg dm−3 AgNO3, which promoted shoots elongation. The genetic stability of the regenerated plants was analyzed by flow cytometry, RAPD and SSR molecular markers. The results indicated that almost no somaclonal variation was detected among the regenerants.read more
Citations
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Organogenesis from root explants of commercial populations of Passiflora edulis Sims and a wild passionfruit species, P. cincinnata Masters
Crislene Viana da Silva,Leandro Silva de Oliveira,Virgilio Adriano Pereira Loriato,Luzimar Campos da Silva,José Marcello Salabert de Campos,Lyderson Facio Viccini,Evelyn Jardim de Oliveira,Wagner Campos Otoni +7 more
TL;DR: Flow cytometric analysis revealed no variation in DNA content of regenerated plantlets among all genotypes, and nuclear DNA (2C) values in regenerants of P. cincinnata and P. edulis were consistent with DNA amounts of seed-derived control plants.
Journal ArticleDOI
Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species
TL;DR: Flow cytometry has been used for plant DNA content estimation since the 1980s; however, presently, the number of laboratories equipped with flow cytometers has significantly increased and these are used extensively not only for research but also in plant breeding and seed production and technology to establish seed maturity, quality and advancement of germination.
Journal ArticleDOI
In vitro regeneration of Anethum graveolens , antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity
Sonali Jana,G. S. Shekhawat +1 more
TL;DR: It has been found that regenerated plants are true to type plants.
Journal ArticleDOI
Visiting eggplant from a biotechnological perspective: A review
TL;DR: This review gathers the information regarding tissue culture, genetic engineering, and genome editing advancements so far accomplished in the eggplant.
Journal ArticleDOI
In vitro propagation and genetic fidelity study of plant regenerated from inverted hypocotyl explants of eggplant (Solanum melongena L.) cv. Arka Shirish.
TL;DR: The regenerated plants generated 96 scorable bands with a total of 1,056 bands for the primers, and comparison of the bands with the mother plant revealed the monomorphic nature and true-to-type clones.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Somaclonal variation — a novel source of variability from cell cultures for plant improvement
Philip J. Larkin,W. R. Scowcroft +1 more
TL;DR: It is argued that this variation in plant cell culture itself generates genetic variability (somaclonal variation) that may be employed to enhance the exchange required in sexual hybrids for the introgression of desirable alien genes into a crop species.
Journal ArticleDOI
Nuclear DNA Amounts in Angiosperms
TL;DR: This paper lists absolute nuclear DNA amounts for 753 angiosperm species, and the dats were assembled primarily for reference purposes, and so the species are listed in alphabetical order, as this was felt to be more helpful to cyto- and biochemists whom, it is anticipated, will be among its major users.
Journal ArticleDOI
Nuclear DNA Amounts in Angiosperms
TL;DR: This paper contains a supplementary list of absolute DNA values, including estimates for 240 angiosperm species not listed by Bennett & Smith in 1976, as well as additional estimates for 41 species already listed by them.
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