Journal ArticleDOI
High efficient prokaryotic expression and purification of bioactive human growth hormone using a cleavable self-aggregating tag
Reads0
Chats0
TLDR
A previously established cleavable self-aggregating tag (cSAT) is employed for the expression and purification of hGH in BL21 (DE3) strain of E. coli to evaluate its effectiveness and offers a straightforward and fast procedure for the production of pure and bioactive hGH.About:
This article is published in Gene Reports.The article was published on 2018-09-01. It has received 4 citations till now. The article focuses on the topics: Intein & Bradford protein assay.read more
Citations
More filters
Journal ArticleDOI
A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
Jiuyuan Bai,Yao Luo,Xin Wang,Shi Li,Mei Luo,Meng Yin,Yuanli Zuo,Guolin Li,Junyu Yao,Hua Yang,Mingdi Zhang,Wei Wei,Maolin Wang,Rui Wang,Chunhai Fan,Yun Zhao +15 more
TL;DR: It is shown that 3WJ-4 × Bro is suitable for transgene identification and as an mRNA reporter for expression pattern analysis and can be used for in vivo dynamic analysis of mRNA.
Journal ArticleDOI
Facile expression and purification of active human growth hormone in E. coli by a cleavable self-aggregating tag scheme.
TL;DR: In this paper, the cleavable selfaggregating tag (cSAT) scheme was used for the expression and purification of recombinant hGH in E. coli, and four aggregating tags (L6KD/α3-peptide/EFK8/ELK16) successfully drove rhGH into active protein aggregates.
Journal ArticleDOI
Efficient biosynthesis of 1-cyanocyclohexaneacetic acid using a highly soluble nitrilase by N-terminus modification of novel peptide tags
TL;DR: The hypothesis that two insertion regions contribute to the unique quaternary structure of the spiral microbial nitrilases, based on cysteine scanning across these two regions, is supported.
Journal ArticleDOI
Soluble Expression, One-Step Purification and Characterization of Recombinant Human Growth Hormone Fused with ompA3 in Escherichia coli
TL;DR: This research provides a feasible and convenient method for the soluble expression of recombinant hGH with correct folding in E. coli, and may lay a foundation for the production and application of hGH in the industry.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Removal of endotoxin from protein solutions by phase separation using Triton X-114.
Yoshitomi Aida,Michael J. Pabst +1 more
TL;DR: This phase separation technique provides a rapid and gentle method for removing endotoxin from protein solutions, and retained normal functions when treating proteins treated by this procedure.
Journal ArticleDOI
Human growth hormone: complementary DNA cloning and expression in bacteria
TL;DR: The data reported predict the previously unknown sequence of the signal peptide of human growth hormone and strengthens the hypothesis that these genes evolved by gene duplication from a common ancestral sequence.
Journal ArticleDOI
Long-Term Safety of Recombinant Human Growth Hormone in Turner Syndrome
TL;DR: The aortic dissection/rupture incidence reflects the higher baseline risk for these events in TS, was consistent with current epidemiological data in smaller TS populations, and is likely unrelated to rhGH.
Journal ArticleDOI
Periplasmic production via the pET expression system of soluble, bioactive human growth hormone
TL;DR: These results demonstrate that the pET expression system is suitable for the rapid and simple isolation of bioactive, soluble hGH from E. coli.