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Journal ArticleDOI

High-Frequency Plant Regeneration from Protoplasts of Indica Rice (Oryza sativa L.) Using Maltose

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TLDR
Of the three basal regeneration media compared, R2 medium was found to be more effective than MS or N6 medium in producing plants and Regeneration frequency increased 8 to 12-fold when maltose was used in place of sucrose as a carbohydrate source.
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This article is published in Journal of Plant Physiology.The article was published on 1993-04-01. It has received 43 citations till now. The article focuses on the topics: Callus & Protoplast.

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BookDOI

Current trends in the embryology of angiosperms.

TL;DR: Zygotic Embryogenesis: Developmental Genetics The Formation of an Embryo from a Fertilized Egg K.B. Downie and R.D. Thomas.
Journal ArticleDOI

Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens

TL;DR: A highly efficient gene transfer method mediated by Agrobacterium tumefaciens was developed for Group I indica rice, which had been quite recalcitrant in tissue culture and transformation.
Journal ArticleDOI

High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress.

TL;DR: In this procedure, about 33% of globular embryos not only developed into the cotyledonary stage but rooted simultaneously, eliminating a separate rooting step, and significant improvement in embryogenesis was achieved.
Journal ArticleDOI

Genotype x culture media interaction effects on regeneration response of three indica rice cultivars

TL;DR: In this paper, the effects of genotypes with callus induction and regeneration media combinations on green plantlet regeneration response were studied for three indica rice (Oryza sativa L.) cultivars, IR-72 and IR-54, and fifteen media combinations involving MS, N6, R2, SK1 and some modifications were tested.
References
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Journal ArticleDOI

A revised medium for rapid growth and bio assays with tobacco tissue cultures

TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI

Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources

TL;DR: In this paper, it was shown that (NH4)2SO4 combined with KNO3 at low concentration is of advantage to the formation, growth and differentiation of pollen callus in rice.
Journal ArticleDOI

The isolation, culture and regeneration of Petunia leaf protoplasts.

TL;DR: Protoplasts isolated from varieties of P. hybrida were found to differ in their cultural requirements, and cell colonies derived from individual mesophyll protoplasts grow rapidly upon subculture, to produce callus capable of shoot differentiation and ultimately whole plant formation.
Journal ArticleDOI

Gene Transfer to Cereals: An Assessment

TL;DR: An assessment of the realities and possibilities of effecting gene transfer to cereal crops and why Agrobacterium has been unsuccessful with cereals is presented.
Journal ArticleDOI

Efficient Plant Regeneration from Rice Protoplasts Through Somatic Embryogenesis

TL;DR: It is reported that plant regeneration can be achieved efficiently and reproducibly from protoplasts of rice isolated from cell suspension cultures.
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