Journal ArticleDOI
High-performance immobilized-metal-ion affinity chromatography of peptides and proteins.
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TLDR
The present status of the undeveloped branch of high-performance immobilized-metal-ion affinity chromatography (HPIMAC) is reviewed and it is demonstrated, high resolution of peptides and protein mixtures can be obtained.About:
This article is published in Journal of Chromatography A.The article was published on 1988-06-29. It has received 72 citations till now. The article focuses on the topics: Ion chromatography & Affinity chromatography.read more
Citations
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Journal ArticleDOI
Immobilized metal ion affinity chromatography
TL;DR: This review covers the principles and practice of IMAC that can be performed under very mild, nondenaturing conditions, and the immobilized metal ion ligand complexes are more likely to withstand wear and tear than are antibodies or enzymes.
Book
Natural Products from Plants
Leland J. Cseke,Ara Kirakosyan,Peter B. Kaufman,Sara L. Warber,James A. Duke,H. L. Brielmann +5 more
TL;DR: Anyone looking for a thorough understanding of the properties of natural plant products, beneficial or harmful, will find it in this volume.
Journal ArticleDOI
Current and prospective applications of metal ion–protein binding
TL;DR: Applications in protein processing are described and variations of immobilized metal ion affinity chromatography are described as well as other metal affinity-based technologies.
Journal ArticleDOI
Immobilized metal ion affinity chromatography: a review on its applications
TL;DR: This review addresses the developments, limitations, and considerations of IMAC in the biopharmaceutical industry.
Journal ArticleDOI
Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications
TL;DR: These articles sum up the existing strategies for method development in CE, especially in the search for generally accepted concepts, but also looking for new, promising reagents and ideas.
References
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Journal ArticleDOI
Intracellular calcium homeostasis
TL;DR: The ctl+ -Transporting Systems of Mitochondria, an analysis of the transport systems of Mit mitochondria, concludes that the systems are efficient and effective at transporting large volumes of material.
Journal ArticleDOI
Metal chelate affinity chromatography, a new approach to protein fractionation
TL;DR: A highly flexible method based on affinities which can be used in a more selective fashion by modern chromatographic techniques is described here.
Journal ArticleDOI
Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatography.
Lennart Andersson,Jerker Porath +1 more
TL;DR: Hen egg albumin (ovalbumin) was fractionated into three components of varying phosphate contents and Porcine pepsin was purified in a similar manner to develop purification procedures for phosphoproteins.
Journal ArticleDOI
Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions.
Jerker Porath,B. Olin +1 more
TL;DR: Specific adsorption is demonstrated for serum proteins on immobilized Ni(II) and Fe(III) and ligand-specific desorption (affinity elution) is demonstrated by including in the buffer system certain solutes which are similar to or identical with some particular amino acids found in proteins.
Journal ArticleDOI
Purification of proteins by IMAC
TL;DR: IMAC, immobilized metal-affinity chromatography, has been used for purification of proteins for a decade and the discussion of structural requirements underlying the metal chelate-protein recognition is focused on.
Related Papers (5)
Metal chelate affinity chromatography, a new approach to protein fractionation
Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions.
Jerker Porath,B. Olin +1 more