Journal ArticleDOI
Immobilized metal ion affinity chromatography: a review on its applications
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TLDR
This review addresses the developments, limitations, and considerations of IMAC in the biopharmaceutical industry.Abstract:
After 35 years of development, immobilized metal ion affinity chromatography (IMAC) has evolved into a popular protein purification technique. This review starts with a discussion of its mechanism and advantages. It continues with its applications which include the purification of histidine-tagged proteins, natural metal-binding proteins, and antibodies. IMAC used in conjunction with mass spectroscopy for phosphoprotein fractionation and proteomics is also covered. Finally, this review addresses the developments, limitations, and considerations of IMAC in the biopharmaceutical industry.read more
Citations
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Importance of the Support Properties for Immobilization or Purification of Enzymes
José C. S. dos Santos,José C. S. dos Santos,Oveimar Barbosa,Claudia Ortiz,Ángel Berenguer-Murcia,Rafael C. Rodrigues,Roberto Fernandez-Lafuente +6 more
TL;DR: This review will focus its attention on the requirements of a support surface to produce the desired objectives, the ideal physical properties of the matrix, the properties ofThe introduced reactive groups, the best surface activation degree to reach the desired objective, and the Properties of the reactive groups will be discussed.
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Challenges and opportunities in the purification of recombinant tagged proteins
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Label-Free Optical Biosensors Based on Aptamer-Functionalized Porous Silicon Scaffolds
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References
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Journal ArticleDOI
Metal chelate affinity chromatography, a new approach to protein fractionation
TL;DR: A highly flexible method based on affinities which can be used in a more selective fashion by modern chromatographic techniques is described here.
Journal ArticleDOI
Instability, stabilization, and formulation of liquid protein pharmaceuticals.
TL;DR: The basic behavior of proteins, their instabilities, and stabilization in aqueous state in relation to the development of liquid protein pharmaceuticals is discussed.
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New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.
TL;DR: A novel nitrilotriacetic acid adsorbent has been prepared for metal chelate affinity chromatography and has been found to chelate Cu2+ and Ni2+ strongly and to be superior to the known iminodiacetic acid Adsorbent.
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Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent
TL;DR: A general purification method for recombinant proteins based upon the selective interaction between a poly-histidine peptide, which is fused to the protein of interest, and a novel metal chelate adsorbent is described.
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Immobilized Gallium(III) Affinity Chromatography of Phosphopeptides
Matthew C. Posewitz,Paul Tempst +1 more
TL;DR: A novel procedure for micropurification of phosphorylated peptides, as a front end to mass spectrometric analysis, is described, and the use of an immobilized metal affinity chromatography (IMAC) in a microtip in combination with Ga(III) ions is proposed.