Journal ArticleDOI
Host range, entry exclusion, and incompatibility of Pseudomonas aeruginosa FP plasmids.
TLDR
The transposons Tn 501, Tn 7, and Tn 1 were used to mark 20 FP plasmids of Pseudomonas aeruginosa which have previously been characterized on the basis of their host chromosome mobilizing ability and their contribution to error-prone DNA repair to study the host range, entry exclusion, and incompatibility systems of these plasmid.About:
This article is published in Plasmid.The article was published on 1980-05-01. It has received 21 citations till now. The article focuses on the topics: Host chromosome & Pseudomonas putida.read more
Citations
More filters
Journal ArticleDOI
The insertion sequence IS21 of R68.45 and the molecular basis for mobilization of the bacterial chromosome.
TL;DR: It is proposed that the chromosome-mobilizing ability of R68.45 is due to the formation of an R.45-chromosome cointegrate during transposition of IS21, which would account for its high efficiency and the absence of a fixed chromosomal origin of transfer in Pseudomonas aeruginosa PAO, and its ability to function in a variety of bacterial hosts.
Journal ArticleDOI
A chromosomally located transposon in Pseudomonas aeruginosa.
M. I. Sinclair,B. W. Holloway +1 more
TL;DR: A new transposon, Tn2521, coding for carbenicillin, streptomycin, spectinomycin and sulfanilamide resistance, has been identified in Pseudomonas aeruginosa and has a pattern of restriction endonuclease sites unlike that of any previously described transposons.
Book ChapterDOI
Broad Host Range Conjugative and Mobilizable Plasmids in Gram-Negative Bacteria
TL;DR: Bacterial conjugation mediates genetic exchange not only between cells of the same species but also between members of distantly related or even unrelated genera, providing a mechanism for the availability of a huge pool of genes for bacterial evolution.
Journal ArticleDOI
Genetic aspects of biodegradation by pseudomonads.
TL;DR: The genetic organization of biodegradation functions in those Pseudomonas species that have been examined in some detail by genetic techniques are discussed and numerous methods that are potentially applicable to the study ofBiodegradation have been developed for Agrobacterium and Rhizobium genetics and some of these will be mentioned.
Journal ArticleDOI
Host range of conjugation and replication functions of the Escherichia coli sex plasmid Flac: Comparison with the broad host-range plasmid RK2
TL;DR: Results show that F lac is able to mediate low frequency transfer to P. aeruginosa, and that the lack of replication in Pseudomonas is ultimately responsible for the restricted host range.
References
More filters
Journal ArticleDOI
DNA Modification and Restriction
TL;DR: The chapter describes that a bacterial strain can protect its own DNA from cleavage by its restriction endonucleases, which is brought about by site-specific methylation of the DNA, for which another activity, the DNA modification methylase, is responsible.
Journal ArticleDOI
Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
TL;DR: Translocatable drug-resistance elements are useful in isolation of mutants, in the construction of strains and other genetic manipulations (even when selection is difficult or impossible), in localized mutagenesis, in chromosomal mapping, in construction of Hfr strains with known origin and direction of chromosome transfer, in complementation tests.
Journal ArticleDOI
Uniform nomenclature for bacterial plasmids: a proposal.
TL;DR: This work aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about the phytochemical properties of E. coli.
Journal ArticleDOI
R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa
Dieter Haas,B. W. Holloway +1 more
TL;DR: Results, combined with linkage studies, indicate that R68.45, unlike the Pseudomonas sex factors FP2 and FP39, promotes chromosome transfer from a range of origin sites and can thus be used for mapping the region of the P. aeruginosa chromosome later than 40 min.
Journal ArticleDOI
Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons.
TL;DR: It is postulate that the TpR SmR segment of R483 is a transposon (TnC) with specific boundary sequences that did not require a functional recA+ gene.
Related Papers (5)
R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa
Dieter Haas,B. W. Holloway +1 more