Journal ArticleDOI
Hypoxia inducible factor-1α expression in areca quid chewing-associated oral squamous cell carcinomas.
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TLDR
Hypoxia inducible factor-1 α expression is significantly upregulated in areca quid chewing-associated OSCC and HIF-1α expression induced by arecoline is downregulated by NAC, curcumin, PD98059, and staurosporine.Abstract:
Oral Diseases (2010) 16, 696–701
Objectives: Hypoxia inducible factor (HIF)-1α gene expression is mainly induced by tissue hypoxia. Overexpression of HIF-1α has been demonstrated in a variety of cancers. The aim of this study was to compare HIF-1α expression in normal human oral epithelium and areca quid chewing-associated oral squamous cell carcinoma (OSCC) and further to explore the potential mechanisms that may lead to induce HIF-1α expression.
Methods: Twenty-five OSCC from areca quid chewing-associated OSCC and 10 normal oral tissue biopsy samples without areca quid chewing were analyzed by immunohistochemistry. The oral epithelial cell line GNM cells were challenged with arecoline, a major areca nut alkaloid, by using Western blot analysis. Furthermore, glutathione precursor N-acetyl-l-cysteine (NAC), AP-1 inhibitor curcumin, extracellular signal-regulated protein kinase inhibitor PD98059, and protein kinase C inhibitor staurosporine were added to find the possible regulatory mechanisms.
Results: Hypoxia inducible factor-1α expression was significantly higher in OSCC specimens than normal specimen (P < 0.05). Arecoline was found to elevate HIF-1α expression in a dose- and time-dependent manner (P < 0.05). The addition of NAC, curcumin, PD98059, and staurosporine markedly inhibited the arecoline-induced HIF-1α expression (P < 0.05).
Conclusions: Hypoxia inducible factor-1α expression is significantly upregulated in areca quid chewing-associated OSCC and HIF-1α expression induced by arecoline is downregulated by NAC, curcumin, PD98059, and staurosporine.read more
Citations
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Curcumin mediates anticancer effects by modulating multiple cell signaling pathways.
Ajaikumar B. Kunnumakkara,Devivasha Bordoloi,Choudhary Harsha,Kishore Banik,Subash C. Gupta,Bharat B. Aggarwal +5 more
TL;DR: The focus of this review is to discuss the molecular basis for the anticancer activities of curcumin based on preclinical and clinical findings.
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Molecular mechanisms of curcumin action: gene expression.
TL;DR: Based on its ability to affect multiple targets, curcumin has the potential for the prevention and treatment of various diseases including cancers, arthritis, allergies, atherosclerosis, aging, neurodegenerative disease, hepatic disorders, obesity, diabetes, psoriasis, and autoimmune diseases.
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Hypoxia-inducible factors in OSCC.
Mario Pérez-Sayáns,José M. Suárez-Peñaranda,Gayoso-Diz Pilar,Francisco Barros-Angueira,José Manuel Gándara-Rey,Abel García-García +5 more
TL;DR: The goal of this paper is to describe HIF basic biology and tumor cells (HIF-1α, mainly), analyzing the effects of its expression in OSCC, study its relation with other molecules such as nitric oxide (NO), carbonic anhydrase (CA) or VEGF and assess the possibility of its manipulation as a therapeutic target.
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Multifaceted Mechanisms of Areca Nuts in Oral Carcinogenesis: the Molecular Pathology from Precancerous Condition to Malignant Transformation.
TL;DR: The up-to-date molecular and cellular mechanisms by which areca nuts contribute to malignant transformation are comprehensively discussed and concisely summarized.
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Identification of upregulated genes in oral squamous cell carcinomas.
Roberta Cardim Lessa,Antonio Campos,Carlos Elias de Freitas,Felipe Rodrigues da Silva,Luiz Paulo Kowalski,André Lopes Carvalho,André Luiz Vettore,André Luiz Vettore +7 more
TL;DR: Upregulated genes could be useful as markers for diagnosis, prognosis, and as new drug targets for head and neck squamous cell carcinomas.
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