Identification and typing of herpes simplex virus by enzyme immunoassay with monoclonal antibodies.
TLDR
A double-antibody enzyme immunoassay was developed by employing a polyclonal rabbit capture antiserum together with type-common and type 2-specific monoclonal antibodies as detectors to identify HSV isolates.Abstract:
A double-antibody enzyme immunoassay was developed for the identification and typing of herpes simplex virus (HSV) by employing a polyclonal rabbit capture antiserum together with type-common and type 2-specific monoclonal antibodies as detectors. The test successfully identified 45 type I isolates and 30 type 2 isolates as HSVs. Compared with immunofluorescent staining and restriction endonuclease analysis, enzyme immunoassay correctly typed 45 type 1 and 30 type 2 HSV isolates. Enzyme immunoassay was 100% sensitive for identification of HSV as compared with cell culture and 100% specific for typing as compared with immunofluorescence and restriction endonuclease analysis. Electron microscopy analysis suggested that approximately 10(6) virus particles were required for the identification and typing of HSV by enzyme immunoassay.read more
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Sample-to-answer microfluidic cartridge
TL;DR: A microfluidic cartridge and methods for performing a diagnostic, molecular or biochemical assay thereon, where all dried and/or liquid reagents necessary for the assay are contained in the cartridge and the assay requires only the addition of sample as discussed by the authors.
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Portable fluorescence detection system and microassay cartridge
Abstract: Disclosed is a compact, microprocessor-controlled instrument for fluorometric assays in liquid samples, the instrument having a floating stage with docking bay for receiving a microfluidic cartridge and a scanning detector head with on-board embedded microprocessor operated under control of a ODAP daemon resident in the detector head for controlling source LEDs, emission signal amplification and filtering in an isolated, low noise, high-gain environment within the detector head. Multiple optical channels may be incorporated in the scanning head. In a preferred configuration, the assay is validated using dual channel optics for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. Applications include molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general, many of which require temperature control during detection.
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Laboratory diagnosis of herpes simplex virus infections. Principles guiding the development of rapid diagnostic tests.
TL;DR: In this article, rapid assays to detect herpes simplex virus using monoclonal and polyclonal antibodies in enzyme-linked immunosorbent assay and immunofluorescent assays and/or detection of HSV deoxyribonucleic acid by hybridization methods have also been developed.
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Fluidic circuits and related manufacturing methods
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References
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Journal ArticleDOI
Comparative Efficacy of Antiherpes Drugs against Different Strains of Herpes Simplex Virus
TL;DR: A large variety of antiherpes compounds was compared for their inhibitory activity against laboratory strains and clinical isolates of herpes simplex virus (HSV) type 1 and type 2, suggesting that phosphorylation by virus-induced TK was required to produce specific inhibition of HSV replication.
Journal ArticleDOI
Infection with herpes-simplex viruses 1 and 2. II.
Andre J. Nahmias,Bernard Roizman +1 more
TL;DR: It is difficult to rule out the possibility that the presence of HSV2 or of antigens is not an independent event unrelated to the cancer, and it is possible that frequent or constant antigenic stimulation occurs in at least some cases of recurrent herpetic infections.
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Risk of recurrence after first episodes of genital herpes. Relation to HSV type and antibody response.
TL;DR: Risk factors associated with recurrent genital herpes-simplex-virus infection caused by either Type 1 or 2 herpesvirus (HSV-1 or HSV-2) were defined and recurrences were more likely to follow an index recurrent episode than an index first episode, whether primary or nonprimary, and were morelikely to occur in men than in women.
Journal ArticleDOI
Growth Inhibition by Acycloguanosine of Herpesviruses Isolated from Human Infections
TL;DR: The antiviral activity of ACG against clinical isolates of HSV-1, HSv-2, and varicella-zoster virus and the lack of toxicity to monkey or human cells in culture at concentrations which markedly inhibited these viruses are demonstrated.
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Demonstration of exogenous genital reinfection with herpes simplex virus type 2 by restriction endonuclease fingerprinting of viral DNA.
Timothy G. Buchman,Timothy G. Buchman,Bernard Roizman,Bernard Roizman,Andre J. Nahmias,Andre J. Nahmias +5 more
TL;DR: Infection with HSV-2 in the same or nearby site can occur in the face of a prior infection with a genetically different strain of the same serotype.