Journal ArticleDOI
Identification of RAPD markers linked to a major rust resistance gene block in common bean.
TLDR
Analysis of a collection of resistant and susceptible cultivars and experimental lines revealed that observations of variable recombination among Mesoamerican bean races suggested suppression of recombination between introgressed segments and divergent recurrent backgrounds.Abstract:
Rust in bean (Phaseolus vulgaris L.), caused byUromyces appendiculatus (Pers.) Unger var.appendiculatus [ =U. phaseoli (Reben) Wint.], is a major disease problem and production constraint in many parts of the world. The predominant form of genetic control of the pathogen is a series of major genes which necessitate the development of efficient selection strategies. Our objective was focused on the identification of RAPD (random amplified polymorphic DNA) markers linked to a major bean rust resistance gene block enabling marker-based selection and facilitating resistance gene pyramiding into susceptible bean germplasm. Using pooled DNA samples of genotyped individuals from two segregating populations, we identified two RAPD markers linked to the gene block of interest. One such RAPD, OF10970 (generated by a 5′-GGAAGCTTGG-3′ decamer), was found to be closely linked (2.15±1.50 centi Morgans) in coupling with the resistance gene block. The other identified RAPD, OI19460 (generated by a 5′-AATGCGGGAG-3′ decamer), was shown to be more tightly linked (also in coupling) than OF10970 as no recombinants were detected among 97 BC6F2 segregating individuals in the mapping population. Analysis of a collection of resistant and susceptible cultivars and experimental lines, of both Mesoamerican and Andean origin, revealed that: (1) recombination between OF10970 and the gene block has occurred as evidenced by the presence of the DNA fragment in several susceptible genotypes, (2) recombination between OI19460 and the gene block has also occurred indicating that the marker is not located within the gene block itself, and (3) marker-facilitated selection using these RAPD markers, and another previously identified, will enable gene pyramiding in Andean germplasm and certain Mesoamerican bean races in which the resistance gene block does not traditionally exist. Observations of variable recombination among Mesoamerican bean races suggested suppression of recombination between introgressed segments and divergent recurrent backgrounds.read more
Citations
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Journal ArticleDOI
Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding
TL;DR: The combination of root growth and morphology traits, phosphorus uptake mechanisms, root acid exudation, and other traits in alleviating phosphorus deficiency, and identification of numerous QTL of relatively minor effect associated with each trait, reveals the complexity to be addressed in breeding for abiotic stress resistance in common bean.
Journal ArticleDOI
Common bean (phaseolus vulgaris l.)
Peter Graham,P. Ranalli +1 more
TL;DR: The origins and general botany of Phaseolus vulgaris is reviewed, the different cropping systems used for bean production, the agronomic and biological factors which most limit crop yield, and the strategies for improvement in this crop.
Journal ArticleDOI
Broadening the Genetic Base of Common Bean Cultivars
TL;DR: The origin, domestication, and organization of genetic diversity in Phaseolus beans is reviewed, production problems and traits deficient in the common bean (P. vulgaris L.) cultivars are highlighted, sources of useful gemplasm are cited, and progress achieved is reviewed.
Journal ArticleDOI
Towards an integrated linkage map of common bean. 4. Development of a core linkage map and alignment of RFLP maps
Rosanna Freyre,Paul W. Skroch,Valérie Geffroy,Anne-Françoise Adam-Blondon,A. Shirmohamadali,W. C. Johnson,V. Llaca,Rubens Onofre Nodari,P. A. Pereira,Siu Mui Tsai,Joe Tohme,M. Dron,J. Nienhuis,C. E. Vallejos,Paul Gepts +14 more
TL;DR: A core linkage map was established in the recombinant inbred population BAT93×Jalo EEP558 (BJ) and comprises 563 markers, including some 120 RFLP and 430 RAPD markers, in addition to a few isozyme and phenotypic marker loci.
Book ChapterDOI
4 – Generating and Using DNA Markers in Plants
TL;DR: This chapter presents description and comparison of different DNA marker systems and suggests that once the mapping parents have been chosen and low-copy RFLP probes obtained, those probes that detect useful polymorphisms in the segregating population must be identified.
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Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
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