Journal ArticleDOI
Immobilization of invertase in conducting copolymers of 3-methylthienyl methacrylate.
TLDR
Kinetic parameters, Michaelis-Menten constant, K(m), and the maximum reaction rate, V(max), were investigated and Operational stability and temperature optimization of the enzyme electrodes were also examined.About:
This article is published in Bioelectrochemistry.The article was published on 2003-04-01. It has received 46 citations till now. The article focuses on the topics: Polypyrrole & Methacrylate.read more
Citations
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Journal ArticleDOI
Enzyme immobilization: an update
TL;DR: This paper is a review of the recent literatures on enzyme immobilization by various techniques, the need for immobilization and different applications in industry, covering the last two decades.
Journal ArticleDOI
Chemically surface modified gel (CSMG): An excellent enzyme-immobilization matrix for industrial processes
TL;DR: Using an optimized immobilization protocol, a very high loading of 723 mg invertase per gram GA-N-CSMG is obtained; 3-200-fold higher than values published in literature.
Journal ArticleDOI
Polyacrylamide–gelatine carrier system used for invertase immobilization
TL;DR: In this article, the effect of polyacrylamide-gelatin ratio and cross-linker concentration on immobilized enzyme activity was analyzed and the optimum conditions, namely substrate concentration, temperature, and pH were determined.
Journal ArticleDOI
Enhanced pH and thermal stabilities of invertase immobilized on montmorillonite K-10
G. Sanjay,S. Sugunan +1 more
TL;DR: In this paper, invertase was adsorbed onto microporous acid-activated montmorillonite clay (K-10) by two procedures, namely adsorption and covalent binding.
Journal ArticleDOI
Biosensing approach for alcohol determination using immobilized alcohol oxidase.
TL;DR: Alcohol oxidase was immobilized in polypyrrole and a random copolymer containing 3-methylthienyl methacrylate and p-vinylbenzyloxy poly(ethyleneoxide) matrices and maximum reaction rates, Michaelis-Menten constants, optimum temperature and pH values, operational stabilities and shelf life of the enzyme electrodes were investigated.
References
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Journal ArticleDOI
The Determination of Enzyme Dissociation Constants
Hans Lineweaver,Dean Burk +1 more
TL;DR: On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, where (E:l = (ES).
Journal ArticleDOI
A photometric adaptation of the somogyi method for the determination of glucose
TL;DR: In this paper, the reliability of the various Somogyi-Shaffer-Hartmann (SHH) copper reagents for glucose determination in biological material has been established, which can be accomplished by omission of the iodide and iodate in their preparation, since these interfere with the molybdate color reagents.
Journal ArticleDOI
Covalent immobilization of invertase onto the surface-modified polyaniline from graft copolymerization with acrylic acid
TL;DR: In this paper, the amide linkage formation between the amino groups of invertase and the carboxyl groups of the grafted AAc polymer chains on emeraldine base form of polyaniline films and powders with surface-grafted acrylic acid (AAc) polymer was investigated.
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Application of immobilized invertase to continuous hydrolysis of concentrated sucrose solutions
Pierre Monsan,Didier Combes +1 more
TL;DR: Invertase immobilized onto corn grits was utilized in the hydrolysis of highly concentrated sucrose solutions producting liquid sugar solutions containing glucose and fructose, indicating that the plug‐flow reactor has an higher efficiency than the continuous stirredtank reactor.
Journal ArticleDOI
Immobilization of invertase in conducting polymer matrices
TL;DR: A novel approach in the electrode immobilization of an enzyme, invertase, by electrochemical polymerization of pyrrole in the presence of enzyme is reported, which demonstrates the effects of pH and temperature on the properties of enzyme electrode.