Journal ArticleDOI
Immunoassay by differential pulse polarography.
TLDR
An immunological method based on labeling an antigen with an electroactive group detectable by differential pulse polarography has been demonstrated and is potentially useful as an analytical immunological technique.Abstract:
An immunological method based on labeling an antigen with an electroactive group detectable by differential pulse polarography has been demonstrated. Estriol labeled with mercuric acetate is electroactive, giving a reduction wave at -300 millivolts versus a standard calomel electrode. Addition of estriol antibody to 4-mercuric acetate estriol diminishes the peak current as a result of the antigen-antibody binding reaction. Separation of free-labeled estriol from antibody-bound-labeled antigen is unnecessary. The method is potentially useful as an analytical immunological technique.read more
Citations
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Journal ArticleDOI
Simultaneous determination of endocrine disrupting phenolic compounds and steroids in water by solid-phase extraction–gas chromatography–mass spectrometry
TL;DR: It is shown that the Oasis cartridges produced the best recoveries of target EDCs while ethyl acetate was efficient in eluting EDCs from SPE cartridges, and the recovery of some EDCs was enhanced by the addition of salt, but reduced by the increase in pH value and humic acid concentration.
Journal ArticleDOI
Strategies for electrochemical immunoassay.
Journal ArticleDOI
Enzyme-linked immunomagnetic electrochemical detection of Salmonella typhimurium
Andrew G. Gehring,C. Gerald Crawford,Ralph S. Mazenko,Lisa J. Van Houten,Jeffrey D. Brewster +4 more
TL;DR: A microbial detection technique that combines the selectivity of antibody-coated superparamagnetic beads with the rapidity and sensitivity of electrochemical detection in a format termed enzyme-linked immunomagnetic electrochemistry is reported.
Journal ArticleDOI
A sensitive determination of estrogens with a Pt nano-clusters/multi-walled carbon nanotubes modified glassy carbon electrode.
Xiangqin Lin,Yongxin Li +1 more
TL;DR: The preliminary study found that this composite modified electrode has strong electrocatalytic activity toward the oxidation of estrogens involving estradiol, estrone and estriol.
Journal ArticleDOI
Voltammetric behavior and determination of estrogens at Nafion-modified glassy carbon electrode in the presence of cetyltrimethylammonium bromide
TL;DR: In this paper, a method for the determination of estrogens (estradiol, estrone and estriol) by stripping voltammetry was described, which yielded one well-defined oxidation peak at the Nafion-modified glassy carbon electrode in the presence of cetyltrimethylammonium bromide.
References
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Journal ArticleDOI
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.
Eva Engvall,Peter Perlmann +1 more
Journal ArticleDOI
Immunoassay of insulin with insulin-antibody precipitate.
C.N. Hales,P.J. Randle +1 more
Book
Textbook of endocrinology
TL;DR: To squeeze all of this into a man¬ ageable volume, the publisher has gone to a smaller type size than before, smaller than usual in medical texts, but the end result is a uniform, informative style.