Journal ArticleDOI
Immunofluorescent Staining of Platelet Suspensions and Detection of Antiplatelet Antibody in Patients with Idiopathic Thrombocytopenic Purpura
TLDR
Direct immunofluorescent staining of platelets obtained from 13 cases of idiopathic thrombocytopenic purpura suggested platelets in patients with ITP may be damaged by an antiplatelet autoantibody acting directly on the platelet surface and/or by antigen antibody complexes binding via Fc IgG receptors on the surface of the platelets.Abstract:
. Direct immunofluorescent staining of 31 specimens of platelets obtained from 13 cases of idiopathic thrombocytopenic purpura (ITP) revealed positive staining on the surface of platelets for both immunoglobulins (Igs) and human β1C globulin in 9 specimens, for only Igs in 1 specimen and for human β1C alone in 5 specimens. The pattern of the positive immunofluorescent staining was granular. Indirect immunofluorescent staining of normal platelets in serum obtained from patients with ITP was positive for antiplatelet antibody in 9 out of 31 specimens. This suggests that platelets in patients with ITP may be damaged by an antiplatelet autoantibody acting directly on the platelet surface and/or by antigen antibody complexes binding via Fc IgG receptors on the surface of the platelets.read more
Citations
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Journal ArticleDOI
A new micromethod for the in vitro detection of antiplatelet antibodies: C-FDA thrombocytotoxicity
G.E. Lizak,F. C. Grumet +1 more
TL;DR: Preliminary data suggest that this method detects possible new non-HLA, non-ABO, nonP1A1 platelet antigens, and was as good or better a clinical test method for all drug-related and isoimmune neonatal thrombocytopenia patient sera tested.
Journal ArticleDOI
Idiopathic thrombocytopenic purpura
Terry R. Burns,Abdus Saleem +1 more
TL;DR: Current methods are becoming highly sensitive and may soon be simple and fast enough for routine clinical use and should significantly aid the diagnosis and management of patients with idiopathic thrombocytopenic purpura.
Journal ArticleDOI
CASE REPORT Vinblastine-loaded platelets–their effect in a patient with immune thrombocytopenia associated with Waldenstrom's macroglobulinaemia
R. Lee,M. S. Rose,C. L. Harmer +2 more
Journal ArticleDOI
A simple method for detecting complement-fixation by autologous platelets in autoimmune thrombocytopenic purpura.
R M Sandler,D Voak,J Darnborough +2 more
TL;DR: A new modification of the microtitre complement fixation test, (CFT), is described for the detection of platelet-bound antibodies (PBA), which was positive in 12 out of 16 patients with active autoimmune thrombocytopenic purpura and in 51 normal blood donors.
Journal ArticleDOI
Antigens for antibodies in idiopathic thrombocytopenic purpura
TL;DR: ITPの 成因に自己抗体が関与す る こ とは, Harringtonが, ITP患 者血漿を正常人に輸 注すると急激な血小板減少が起こることを証明 し,
References
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Journal ArticleDOI
Preparation of Suspensions of Washed Platelets from Humans
TL;DR: Methods have been developed for the preparation of suspensions of washed platelets from humans and platelets suspended in Eagle's tissue culture medium containing albumin were more responsive to ADP than platelets in Tyrode's‐albumin solution.
Journal ArticleDOI
Quantitative determination of antibody in idiopathic thrombocytopenic purpura. Correlation of serum and platelet-bound antibody with clinical response.
TL;DR: Quantitation of surface IgG of thrombocytopenic platelets was useful in predicting response to treatment and correlated with failure to respond to prednisone therapy.
Journal ArticleDOI
Similarities between known antiplatelet antibodies and the factor responsible for thrombocytopenia in idiopathic purpura. physiologic, serologic and isotopic studies
TL;DR: Direct evidence is concerns direct evidence that the ITP factor destroys autologous platelets and is most likely an antibody.
Journal ArticleDOI
Idiopathic Thrombocytopenic Purpura
TL;DR: The old hypothesis, originally postulated by Frank, of a toxic depression of megakaryocytes and their activity resulting in thrombocytopenia has been disproved since a shortened platelet life-span has regularly been found in the disease.
Journal ArticleDOI
In vitro detection of platelet antibody in patients with idiopathic thrombocytopenic purpura and systemic lupus erythematosus.
Simon Karpatkin,G W Siskind +1 more
TL;DR: Two technics have been developed, which make possible the in vitro detection of platelet antibody in patients with idiopathic thrombocytopenic purpura, and systemic lupus erythematosus, and the antiplatelet factor was found to be an immunoglobulin of the IgG class.
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